CHD7 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogen
CHD7 antibody was raised against a 13 amino acid synthetic peptide near the internal region of CHD7.
CHD7
Reaktivität: Human
WB, ICC
Wirt: Schaf
Polyclonal
unconjugated
Applikationshinweise
ELISA: Antibody detection limit dilution 1:64,000. Western Blot: Approximately 250 kDa and 48 kDa in rat spinal cord lysates after 1 μg/mL antibody staining. Both detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands).
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Liquid
Konzentration
500 μg/mL
Buffer
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Konservierungsmittel
Sodium azide
Vorsichtsmaßnahmen
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handhabung
Minimize freezing and thawing.
Lagerung
-20 °C
Informationen zur Lagerung
Aliquot and store at -20 °C.
Target
CHD7
(Chromodomain Helicase DNA Binding Protein 7 (CHD7))