This antibody was raised against the C-terminus of NP_055210.1. However, NCBI has changed the version to NP_055210.2 thus losing the C-terminal epitope.
Aufreinigung
Antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogen
DAPP1 antibody was raised against a 14 amino acid synthetic peptide near the C-Terminus of DAPP1.
DAPP1
Reaktivität: Human
WB
Wirt: Maus
Polyclonal
unconjugated
Applikationshinweise
ELISA: Antibody detection limit dilution 1:4000. Western Blot: Approximately 26 kDa band in human heart lysates after 1 μg/mL antibody staining.0 kDa according to AAF14578.1. The 26 kDa band was successfully blocked by incubation with the immunizing peptide. Approximately 30 kDa band observed in human kidney lysates (calculated MW of 32.0 kDa according to AAF14578.1). Recommended concentration: 0.5-1 μg/mL.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Liquid
Konzentration
500 μg/mL
Buffer
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Konservierungsmittel
Sodium azide
Vorsichtsmaßnahmen
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handhabung
Minimize freezing and thawing.
Lagerung
-20 °C
Informationen zur Lagerung
Aliquot and store at -20 °C.
Target
DAPP1
(Dual Adaptor of Phosphotyrosine and 3-phosphoinositides (DAPP1))