Dieser Serotonin Receptor 1A Antikörper ist unkonjugiert
Applikation
Western Blotting (WB), ELISA
Aufreinigung
Htr1a antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogen
Htr1a antibody was raised against a 13 amino acid synthetic peptide near the internal region of Htr1a (mouse).
ELISA: Antibody detection limit dilution 1:128000. Western Blot: Approximately 45 kDa band observed in mouse brain lysates (calculated MW of 46.2 kDa according to mouse NP_032334.2). Recommended concentration: 1-3 μg/mL. Additional bands of 75 and 18 kDa of unknown identity were consistently observed. These bands were successfully blocked by incubation with the immunizing peptide.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Liquid
Konzentration
500 μg/mL
Buffer
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Konservierungsmittel
Sodium azide
Vorsichtsmaßnahmen
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.