Western Blotting (WB), Immunoprecipitation (IP), ELISA
Aufreinigung
TRPC7 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogen
TRPC7 antibody was raised against a 13 amino acid synthetic peptide near the internal region of TRPC7.
TRPC7
Reaktivität: Human
WB, IP, IHC, IF, ICC, AA
Wirt: Maus
Monoclonal
N64A-36 (Formerly S64A-36)
Atto 594
Applikationshinweise
ELISA: Antibody detection limit dilution 1:32000. Western Blot: Approximately 20 kDa band in lysates of cell lines NIH3T3 and KELLY after 1 μg/mL antibody staining.6 kDa according to NP_065122.1. The 20 kDa band was successfully blocked by incubation with the immunizing peptide.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Liquid
Konzentration
500 μg/mL
Buffer
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Konservierungsmittel
Sodium azide
Vorsichtsmaßnahmen
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.