Zona Pellucida Glycoprotein 3 Antikörper

Produktnummer ABIN2461230

Produktdetails anti-Zona Pellucida Glycoprotein 3 Antikörper

Target: Zona Pellucida Glycoprotein 3 (ZP3)

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Zona Pellucida Glycoprotein 3 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA

Aufreinigung: Antibody is purified by protein A chromatography method.

Immunogen: Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human ZP3.

Anwendungsinformationen

Applikationshinweise: ZP3 antibody can be used for detection of ZP3 by ELISA at 1:62500. ZP3 antibody can be used for detection of ZP3 by western blot at 0.6 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 100 ?L of distilled water. Final antibody concentration is 1 mg/mL.

Konzentration: 1 mg/mL

Buffer: Antibody is lyophilized in PBS buffer with 2 % sucrose.

Handhabung: As with any antibody avoid repeat freeze-thaw cycles.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: For short periods of storage (days) store at 4 °C. For longer periods of storage, store ZP3 antibody at -20 °C.

Details zu Zona Pellucida Glycoprotein 3

Target: Zona Pellucida Glycoprotein 3 (ZP3)

Andere Bezeichnung: ZP3 (ZP3 Produkte)

Synonyme: ZP3A antikoerper, ZP3B antikoerper, ZPC antikoerper, Zp-3 antikoerper, zp3.2 antikoerper, Zp-3B antikoerper, zona pellucida glycoprotein 3 antikoerper, zona pellucida sperm-binding protein 3 antikoerper, ZP3 antikoerper, zp3 antikoerper, Zp3 antikoerper

Hintergrund: The zona pellucida is an extracellular matrix that surrounds the oocyte and early embryo. It is composed primarily of three or four glycoproteins with various functions during fertilization and preimplantation development. The protein encoded by this gene is a structural component of the zona pellucida and functions in primary binding and induction of the sperm acrosome reaction. The nascent protein contains a N-terminal signal peptide sequence, a conserved ZP domain, a C-terminal consensus furin cleavage site, and a transmembrane domain. It is hypothesized that furin cleavage results in release of the mature protein from the plasma membrane for subsequent incorporation into the zona pellucida matrix. However, the requirement for furin cleavage in this process remains controversial based on mouse studies. A variation in the last exon of this gene has previously served as the basis for an additional ZP3 locus, however, sequence and literature review reveals that there is only one full-length ZP3 locus in the human genome. Another locus encoding a bipartite transcript designated POMZP3 contains a duplication of the last four exons of ZP3, including the above described variation, and maps closely to this gene

Molekulargewicht: 47 kDa, 23 kDa

Gen-ID: 7784

Pathways: Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process