HLA-F Antikörper

Produktnummer ABIN2459284

Produktdetails anti-HLA-F Antikörper

Target: HLA-F (HLAF) (HLA Class I alpha F (HLAF))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser HLA-F Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA

Aufreinigung: Antibody is purified by peptide affinity chromatography method.

Immunogen: Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human HLA-F.

Anwendungsinformationen

Applikationshinweise: HLA-F antibody can be used for detection of HLA-F by ELISA at 1:12500. HLA-F antibody can be used for detection of HLA-F by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 50 ?L of distilled water. Final antibody concentration is 1 mg/mL.

Konzentration: 1 mg/mL

Buffer: Antibody is lyophilized in PBS buffer with 2 % sucrose.

Handhabung: As with any antibody avoid repeat freeze-thaw cycles.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: For short periods of storage (days) store at 4 °C. For longer periods of storage, store HLA-F antibody at -20 °C.

Details zu HLA-F

Target: HLA-F (HLAF) (HLA Class I alpha F (HLAF))

Andere Bezeichnung: HLA-F (HLAF Produkte)

Synonyme: HLAF antikoerper, CDA12 antikoerper, HLA-5.4 antikoerper, HLA-CDA12 antikoerper, major histocompatibility complex, class I, F antikoerper, HLA-F antikoerper

Hintergrund: HLA-F belongs to the HLA class I heavy chain paralogues. It is a non-classical heavy chain that forms a heterodimer with a beta-2 microglobulin light chain, with the heavy chain anchored in the membrane. Unlike most other HLA heavy chains, this molecule is localized in the endoplasmic reticulum and Golgi apparatus, with a small amount present at the cell surface in some cell types. It contains a divergent peptide-binding groove, and is thought to bind a restricted subset of peptides for immune presentation. This gene belongs to the HLA class I heavy chain paralogues. It encodes a non-classical heavy chain that forms a heterodimer with a beta-2 microglobulin light chain, with the heavy chain anchored in the membrane. Unlike most other HLA heavy chains, this molecule is localized in the endoplasmic reticulum and Golgi apparatus, with a small amount present at the cell surface in some cell types. It contains a divergent peptide-binding groove, and is thought to bind a restricted subset of peptides for immune presentation. This gene exhibits few polymorphisms. Multiple transcript variants encoding different isoforms have been found for this gene. These variants lack a coding exon found in transcripts from other HLA paralogues due to an altered splice acceptor site, resulting in a shorter cytoplasmic domain.

Molekulargewicht: 48 kDa

Gen-ID: 3134

NCBI Accession: NP_001091949

UniProt: P30511

Pathways: Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process