WT1 Antikörper

Produktnummer ABIN2458289

Produktdetails anti-WT1 Antikörper

Target: WT1 (Wilms Tumor 1 (WT1))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser WT1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA

Aufreinigung: Antibody is purified by peptide affinity chromatography method.

Immunogen: Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human WT1.

Anwendungsinformationen

Applikationshinweise: WT1 antibody can be used for detection of WT1 by ELISA at 1:312500. WT1 antibody can be used for detection of WT1 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 50 ?L of distilled water. Final antibody concentration is 1 mg/mL.

Konzentration: 1 mg/mL

Buffer: Antibody is lyophilized in PBS buffer with 2 % sucrose.

Handhabung: As with any antibody avoid repeat freeze-thaw cycles.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: For short periods of storage (days) store at 4 °C. For longer periods of storage, store WT1 antibody at -20 °C.

Details zu WT1

Target: WT1 (Wilms Tumor 1 (WT1))

Andere Bezeichnung: WT1 (WT1 Produkte)

Synonyme: AWT1 antikoerper, EWS-WT1 antikoerper, GUD antikoerper, NPHS4 antikoerper, WAGR antikoerper, WIT-2 antikoerper, WT33 antikoerper, CWT1 antikoerper, WT1 antikoerper, XWT1a antikoerper, wt-1 antikoerper, wt1 antikoerper, xWT1 antikoerper, WT-1 antikoerper, gud antikoerper, wagr antikoerper, wt33 antikoerper, xwt1 antikoerper, wit-2 antikoerper, si:ch211-160j6.3 antikoerper, zgc:152697 antikoerper, D630046I19Rik antikoerper, Wt-1 antikoerper, XWT1b antikoerper, XeWT1 antikoerper, Wilms tumor 1 antikoerper, Wilms tumor 1 S homeolog antikoerper, wilms tumor 1b antikoerper, Wilms tumor protein antikoerper, Wilms tumor 1 homolog antikoerper, Wilms tumor 1 L homeolog antikoerper, WT1 antikoerper, wt1.S antikoerper, wt1 antikoerper, wt1b antikoerper, Wt1 antikoerper, wt1.L antikoerper

Hintergrund: WT1 is a transcription factor that contains four zinc-finger motifs at the C-terminus and a proline/glutamine-rich DNA-binding domain at the N-terminus. It has an essential role in the normal development of the urogenital system, and it is mutated in a small subset of patients with Wilm's tumors. Multiple transcript variants, resulting from alternative splicing at two coding exons, have been well characterized. There is also evidence for the use of non-AUG (CUG) translation initiation site upstream of, and in-frame with the first AUG, leading to additional isoforms.This gene encodes a transcription factor that contains four zinc-finger motifs at the C-terminus and a proline/glutamine-rich DNA-binding domain at the N-terminus. It has an essential role in the normal development of the urogenital system, and it is mutated in a small subset of patients with Wilm's tumors. Multiple transcript variants, resulting from alternative splicing at two coding exons, have been well characterized. There is also evidence for the use of non-AUG (CUG) translation initiation site upstream of, and in-frame with the first AUG, leading to additional isoforms. Authors of PMID:7926762 also provide evidence that WT1 mRNA undergoes RNA editing in human and rat, and that this process is tissue-restricted and developmentally regulated.

Molekulargewicht: 56 kDa

Gen-ID: 7490

NCBI Accession: NP_077742

UniProt: P19544

Pathways: Tube Formation