Ubiquitin Antikörper

Produktnummer ABIN2192145

Produktdetails anti-Ubiquitin Antikörper

Target: Ubiquitin

Reaktivität: Chemical

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Ubiquitin Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunoassay (IA)

Sterilität: 0.2 μm filtered

Klon: Ubi-1

Anwendungsinformationen

Applikationshinweise: For immunohistology, immunofluorescence and Western blotting dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:10. For Western blotting it is recommended to use lysis buffer in 10 mM N-ethylmaleimide (e.g. Pierce #23030) to inhibit ubiquitin-conjugating enzymes. N-ethylmaleimide inactivates certain enzymes by blocking free sulfhydryls. After eletrophoresis and transfer, pre-incubate transferred membranes in denaturing buffer (6 M guanidine-HCl, 20 mM Tris-HCl, 5 mM beta-mercaptoethanol, 1 mM PMSF ( pH 7.5)) for 30-60 minutes at 4 °C, followed by extensive PBS washing.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Buffer: PBS, containing 0.02 % sodium azide and 0.1 % bovine serum albumin.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Product should be stored at 4 °C. Under recommended storage conditions, product is stable for one year.

Haltbarkeit: 12 months

Referenzen für anti-Ubiquitin Antikörper (ABIN2192145)

Holbert, Denghien, Kiechle, Rosenblatt, Wellington, Hayden, Margolis, Ross, Dausset, Ferrante, Néri: "The Gln-Ala repeat transcriptional activator CA150 interacts with huntingtin: neuropathologic and genetic evidence for a role in Huntington's disease pathogenesis." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 98, Issue 4, pp. 1811-6, (2001) (PubMed).

Mullally, Moos, Edes, Fitzpatrick: "Cyclopentenone prostaglandins of the J series inhibit the ubiquitin isopeptidase activity of the proteasome pathway." in: The Journal of biological chemistry, Vol. 276, Issue 32, pp. 30366-73, (2001) (PubMed).

Details zu Ubiquitin

Target: Ubiquitin

Andere Bezeichnung: Ubiquitin (Ubiquitin Produkte)

Synonyme: CG11624 antikoerper, DmUb antikoerper, DmUbi-p63E antikoerper, Dmel\\CG11624 antikoerper, Ub antikoerper, Ubi-P63e antikoerper, Ubi-p antikoerper, Ubi63E antikoerper, Ubi63p antikoerper, Ubip antikoerper, Ubiq antikoerper, Ubp antikoerper, ubi antikoerper, ubi-p63E antikoerper, ubi63E antikoerper, CG18282 antikoerper, CG32744 antikoerper, CR32744 antikoerper, DmUbi-p5E antikoerper, Dmel\\CG32744 antikoerper, ubiquitin antikoerper, si:dz180g5.5 antikoerper, si:busm1-180g5.5 antikoerper, LOC100194618 antikoerper, UBIQ antikoerper, Ubiquitin antikoerper, LOC100282068 antikoerper, Ubiquitin-63E antikoerper, Ubiquitin-5E antikoerper, transmembrane and ubiquitin-like domain containing 2 antikoerper, polyubiquitin antikoerper, Putative ubiquitin family protein antikoerper, ubiquitin antikoerper, ubiquitin B antikoerper, UBiQuitin antikoerper, Ubiquitin antikoerper, Ubi-p63E antikoerper, Ubi-p5E antikoerper, tmub2 antikoerper, LOC100194618 antikoerper, pco110026 antikoerper, LOC100135798 antikoerper, UBB antikoerper, ubq-1 antikoerper, LOC100190766 antikoerper

Substanzklasse: Chemical

Hintergrund: The monoclonal antibody Ubi-1 recognizes ubiquitin, both conjugated and unconjugated. Ubiquitin is a highly conserved globular 76 amino acid protein of about 8.5 kDa. It has an important role in the targeting of proteins for proteolytic degradation. Proteins to be degraded are covalently coupled to the C-terminus of ubiquitin by means of ubiquitin ligases. The ubiquitin itself is frequently also ubiquitinated, producing a polyubiquitin chain. The polyubiquitinated complex is then recognized by a complex of degradative enzymes which form the proteosome. Ubiquitin is also covalently attached to a variety of other pathological inclusions seen in human diseases which appear to be resistant to normal degradation. These inclusions include the neurofibrillary tangles of Alzheimer's disease and progressive supranuclear palsy, Pick bodies of Pick's disease, Lewy bodies of Parkinson's disease, Mallory bodies of alcoholic liver disease, Rosenthal fibers of Alexander's disease, and the inclusion bodies in inclusion myositis and oculopharyngeal muscular dystrophy. The ubiquitin molecule appears to be present in all eukaryotic cells and has an identical primary structure in all animals. Reactivity has been shown in human, bovine, chicken, Drosophilia and C. elegans. Ubiquitin is present in the nucleus, cytoplasm and on cell surface membranes. The monoclonal antibody Ubi-1 was raised against purified bovine ubiquitin conjugated with glutaraldehyde to keyhole limpet hemocyanin.

Pathways: Mitotic G1-G1/S Phases, Ubiquitin Proteasome Pathway