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Siglec H Antikörper (FITC) Primary Antibody

Reaktivität: Maus IHC (fro), FACS Wirt: Ratte Monoclonal 440c FITC
Pubmed (1 reference)
Produktnummer ABIN2191825
$813.93
Zzgl. Versandkosten $45.00
100 μg
Lieferung in 6 bis 8 Werktagen
  • Target See all Siglec H products
    Siglec H
    Reaktivität
    Maus
    Wirt
    • 7
    Ratte
    Klonalität
    • 7
    Monoklonal
    Konjugat
    • 4
    • 2
    • 1
    Dieser Siglec H Antikörper ist konjugiert mit FITC
    Applikation
    • 4
    • 3
    • 3
    • 1
    • 1
    • 1
    Immunohistochemistry (Frozen Sections) (IHC (fro)), Flow Cytometry (FACS)
    Sterilität
    0.2 μm filtered
    Klon
    440c
  • Applikationshinweise
    For immunohistology and flow cytometry dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50.
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Buffer
    PBS, containing 0.02 % sodium azide and 1 % bovine serum albumin.
    Vorsichtsmaßnahmen
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Lagerung
    4 °C
    Informationen zur Lagerung
    Product should be stored at 4 °C. Under recommended storage conditions, product is stable for one year.
    Haltbarkeit
    12 months
  • Blasius, Vermi, Krug, Facchetti, Cella, Colonna: "A cell-surface molecule selectively expressed on murine natural interferon-producing cells that blocks secretion of interferon-alpha." in: Blood, Vol. 103, Issue 11, pp. 4201-6, (2004) (PubMed).

  • Target
    Siglec H
    Andere Bezeichnung
    Siglec-H (Siglec H Produkte)
    Synonyme
    6430529G09Rik, Siglec-H, sialic acid binding Ig-like lectin H, Siglech
    Hintergrund
    The monoclonal antibody 440c reacts with Siglec-H, a cell-surface receptor molecule selectively expressed on murine natural interferon producing cells (IPC), also called mouse plasmacytoid dendritic cells (pDC). Siglec H is unique among Sialic acid-binding Ig-like lectins (Siglecs) proteins because it associates with the adaptor protein DAP12. DAP12 recognize certain viruses and CpG-DNA through TLR9, resulting in secretion of IFN-alpha, IL-12 and proinflammatory chemokines. Together these cytokines and chemokines recruite and activate NK cells and T cells as well as modulating the antigen presenting function of dendritic cells (DC). IPC themselves also function as antigen presenting cells that expand memory T cells and induce Th1 differentiation. Therefore IPC may provide a first line of host defense against viral infections by activating both innate and adaptive responses in vivo. The monoclonal antibody 440c exclusively recognizes mouse Siglec-H in all lymphoid organs under both normal and inflammatory conditions. Siglec-H is normal present in the T cell zone of lymph nodes and spleen. Incubation of IPC with antibody 440c in vitro or administration of antibody 440c in vivo reduces secretion of IFN-alpha in response to CpG DNA without causing IPC depletion.
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