Angiopoietin 1 Antikörper (N-Term)

Produktnummer ABIN218573

Produktdetails anti-Angiopoietin 1 Antikörper

Target: Angiopoietin 1 (ANGPT1)

Bindungsspezifität: N-Term

Reaktivität: Human, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Angiopoietin 1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Marke: IHC-plus™

Spezifität: Synthetic peptide N-Q-R-R-N-P-E-N-G-G-R-R-Y-N-R-I-Q-H-G-Q corresponding to a region (aa 21-40) near the N-terminus of mouse angiopoietin 1 protein conjugated to KLH using maleimide. A residue of cysteine was added to the amino terminal end to facilitate coupling.

Aufreinigung: Sterile filtered

Immunogen: Synthetic peptide from mouse ANGPT1 / Angiopoietin-1.

Type of Immunogen: Synthetic peptide

Anwendungsinformationen

Applikationshinweise: Approved: ELISA (1:5000 - 1:25000), IHC, IHC-P (1:200), WB (1:500 - 1:2000)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues and mouse tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 1:200.

Kommentare:

Target Species of Antibody: Mouse

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: 0.02 M potassium phosphate, 0.15 M sodium chloride,  pH 7.2, 0.01 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeat freeze-thaw cycles.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Details zu Angiopoietin 1

Target: Angiopoietin 1 (ANGPT1)

Andere Bezeichnung: ANGPT1 / ANG1 / Angiopoietin-1 (ANGPT1 Produkte)

Synonyme: AGP1 antikoerper, AGPT antikoerper, ANG1 antikoerper, 1110046O21Rik antikoerper, Ang-1 antikoerper, Ang1 antikoerper, Agpt antikoerper, Agpt1 antikoerper, ang1 antikoerper, wu:fc76g06 antikoerper, ANG-1 antikoerper, angpt1 antikoerper, MGC80788 antikoerper, angiopoietin 1 antikoerper, angiopoietin 1 S homeolog antikoerper, ANGPT1 antikoerper, Angpt1 antikoerper, angpt1 antikoerper, angpt1.S antikoerper

Hintergrund: Name/Gene ID: ANGPT1

Synonyms: ANGPT1, Angiopoietin 1, Angiopoietin-1, AGPT, AGP1, ANG-1, ANG1, KIAA0003

Gen-ID: 284

UniProt: Q15389

Pathways: RTK Signalweg, Glycosaminoglycan Metabolic Process