mGluR2/3 Antikörper (AA 871-883)

Produktnummer ABIN215162

Produktdetails anti-mGluR2/3 Antikörper

Target: mGluR2/3 (GRIA2/3) (Glutamate Receptor, Ionotropic, AMPA 2/3 (GRIA2/3))

Bindungsspezifität: AA 871-883

Reaktivität: Human, Ratte, Maus, Huhn, Zebrafisch (Danio rerio), Pferd, Affe, Fledermaus, Kaninchen, Rind (Kuh), Hund, Xenopus laevis, Hamster

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser mGluR2/3 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Frozen Sections) (IHC (fro))

Spezifität: Recognizes both GluR2 and GluR3 which have nearly identical carboxy terminal sequences. Western blot analysis of rat brain shows two poorly separated bands which co-migrate with GluR2 and GluR3 expressed in transfected cells. GluR2 migrates slightly ahead of GluR3. No cross-reaction with GluR1 or GluR4. Species Reactivity: Human, rat, monkey, gerbil and hamster.

Homologie: Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Bovine, Dog, Bat, Horse, Rabbit, Opossum, Zebra finch, Chicken, Platypus, Xenopus, Pufferfish, Zebrafish (100%) Turkey, Stickleback, Trachemys (92%).

Aufreinigung: Affinity purified

Immunogen: Carboxy terminus peptide of rat GluR2 conjugated to BSA with glutaraldehyde (EGYNVYGIESVKI). Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Bovine, Dog, Bat, Horse, Rabbit, Opossum, Zebra finch, Chicken, Platypus, Xenopus, Pufferfish, Zebrafish (100%), Turkey, Stickleback, Trachemys (92%).

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Approved: IHC, IHC-Fr, WB

Usage: Can be used for immunohistochemistry using paraformaldehyde or paraformaldehyde/glutaraldehyde fixed tissue with light and electron microscopy. Cryostat and vibratome sections can be used with or without Triton X-100 treatment. Final concentrations used were 1-3 μg/mL. Western blot analysis can be done at final concentrations of 0.1-1.0 μg/mL. Labels a band at approximately 108kD. The antibody linked to immobilized Protein A immunoprecipitates detergent-solubilized receptor from brain or transfected cells.

Kommentare:

Target Species of Antibody: Rat

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: deionized water

Konzentration: Lot specific

Buffer: Lyophilized.

Handhabung: Avoid repeat freeze-thaw cycles.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Details zu mGluR2/3

Target: mGluR2/3 (GRIA2/3) (Glutamate Receptor, Ionotropic, AMPA 2/3 (GRIA2/3))

Andere Bezeichnung: GLUR2 + GLUR3 (GRIA2/3 Produkte)

Synonyme: glutamate ionotropic receptor AMPA type subunit 2 antikoerper, GRIA2 antikoerper

Hintergrund: This antibody detects: GLUR2 + GLUR3

UniProt: P42262