FCGR1A Antikörper (FITC)

Produktnummer ABIN2144676

Produktdetails anti-FCGR1A Antikörper (FITC)

Target: FCGR1A (Fc Fragment of IgG, High Affinity Ia, Receptor (CD64) (FCGR1A))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser FCGR1A Antikörper ist konjugiert mit FITC

Applikation: Flow Cytometry (FACS), Western Blotting (WB), Immunocytochemistry (ICC), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Produktmerkmale: 4A10.1 reacts with high affinity receptor for IgG (FcyRI), a 75 kDa type 1 trasmembrane glycoprotein. CD64 is expressed on monocytes and macrophages but not on lymphocytes or resting granulocytes. CD64 play a role in phagocytosis, and antibody dependent cellular cytotoxicity ( ADCC). It also participates in cytokine and superoxide release.

Klon: 4A10-1

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Testdurchführung:

• Take 100 μL peripheral blood anticoagulated by EDTA and add to the bottom of 5 mLtube,
• Add 10 μL labeled antibody to the bottom of flow tube mixing with the whole blood, incubate for 20 minutes at room temperature away from light,
• Add 2 ml1×RBC lysis buffer, incubate for 10 minutes away from light after mixing, dissolve red blood cells (recommended: RBC lysing Solution 10×,Cat.: FXP001),
• Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant,
• Add 2 mLPBS wash buffer to resuspend the cells, then1000 rpm centrifugation for 5 minutes, discard the supernatant,
• Add 0.5 mLPBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 °C then measured).
• [PBS wash buffer: PBS +1 % FBS +0.1 % NaN3, Cat.: FXP005]
• [Cell fixation: 2 % formaldehyde solution]

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: Phosphate-buffered solution, pH 7.4, containing and 0.2 % (w/v) BSA

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Details zu FCGR1A

Target: FCGR1A (Fc Fragment of IgG, High Affinity Ia, Receptor (CD64) (FCGR1A))

Andere Bezeichnung: CD64 (FCGR1A Produkte)

Synonyme: CD64 antikoerper, CD64A antikoerper, FCRI antikoerper, IGFR1 antikoerper, FcgammaRI antikoerper, Fcgr1 antikoerper, Fcgr1b antikoerper, AI323638 antikoerper, AV092959 antikoerper, IGGHAFC antikoerper, FCGR1A antikoerper, Fc fragment of IgG receptor Ia antikoerper, Fc receptor, IgG, high affinity I antikoerper, FCGR1A antikoerper, Fcgr1a antikoerper, Fcgr1 antikoerper

Pathways: Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process