BUB1 Antikörper (AA 281-419)

Produktnummer ABIN199933

Produktdetails anti-BUB1 Antikörper

Target: BUB1 (Budding Uninhibited By Benzimidazoles 1 Homolog (Yeast) (BUB1))

Bindungsspezifität: AA 281-419

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser BUB1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Immunoprecipitation (IP)

Spezifität: Amino acid residues 281-419 of human BUB1 protein.

Aufreinigung: Protein A purified

Immunogen: Recombinant human Bub1.

Type of Immunogen: Recombinant protein

Anwendungsinformationen

Applikationshinweise: Approved: IF, IP, WB

Usage: This protein A purified antibody has been tested for use in immunoprecipitation, immunofluorescence staining and western blot and is capable of detecting endogenous protein. Specific conditions for reactivity should be optimized by the end user. Expect a predominant band at ~160 kD corresponding to full-length protein by western blotting in the appropriate cell lysate or extract. The use of HeLa whole cell lysates prepared using a RIPA buffer is recommended as a positive control. For IF microscopy use cells grown on cover slips fixed with 3.5 % paraformaldehyde in PBS at  pH 6.8 OR 100 % methanol at -20? C. Permeabilize fixed cells with 0.5 % Triton X-100.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: 0.02 M potassium phosphate, 0.15 M sodium chloride,  pH 7.2, 0.01 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid cycles of freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Store vial at -20°C prior to opening. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20°C or below. Avoid freeze-thaw cycles.

Details zu BUB1

Target: BUB1 (Budding Uninhibited By Benzimidazoles 1 Homolog (Yeast) (BUB1))

Andere Bezeichnung: BUB1 (BUB1 Produkte)

Synonyme: bub1a antikoerper, bub1l antikoerper, hbub1 antikoerper, xbub1 antikoerper, BUB1A antikoerper, BUB1L antikoerper, hBUB1 antikoerper, AL022991 antikoerper, Bub1a antikoerper, C80208 antikoerper, D2Xrf87 antikoerper, BUB1 mitotic checkpoint serine/threonine kinase antikoerper, BUB1, mitotic checkpoint serine/threonine kinase antikoerper, BUB1 antikoerper, bub1 antikoerper, Bub1 antikoerper

Hintergrund: Name/Gene ID: BUB1
Subfamily: BUB1
Family: Protein Kinase

Synonyms: BUB1, Bub1A, BUB1L, HBUB1

Gen-ID: 699

UniProt: O43683