MEK1 Antikörper (Ser221)

Produktnummer ABIN197388

Dieses Anti-MEK1-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von MEK1 in WB und IHC (p). Geeignet für Human und Maus.

Kurzübersicht für MEK1 Antikörper (Ser221) (ABIN197388)

Target: MEK1 (MAP2K1) (Mitogen-Activated Protein Kinase Kinase 1 (MAP2K1))

Reaktivität: Human, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser MEK1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-MEK1 Antikörper

Bindungsspezifität: Ser221

Spezifität: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. MEK1 antibody AP02693PU detects endogenous levels of total MEK1 protein.

Aufreinigung: Immunoaffinity chromatography.

Immunogen: The antiserum was produced against synthesized non-phosphopeptide derived from human MEK1 around the phosphorylation site of serine 221 (A-N-Sp-F-V).

Anwendungsinformationen

Applikationshinweise: Suitable for use in in Western blot (1: 500-1: 1000) and Immunohistochemistry (1: 50-1: 100).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % Sodium Azide and 50 % Glycerol.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Store the antibody (in aliquots) at -20 °C.

Details zu MEK1

Target: MEK1 (MAP2K1) (Mitogen-Activated Protein Kinase Kinase 1 (MAP2K1))

Andere Bezeichnung: MAP2K1

Hintergrund: MEK1 (Mitogen activated protein kinase kinase 1) catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in a Thr-Glu-Tyr sequence located in MAP kinases. MEK1 activates ERK1 and ERK2 MAP kinases. MEK1 is activated by dual phosphorylation, which occurs on serine 218 and 222, in the activation loop of the MEK. Threonine 292 of MEK1 is phosphorylated by ERK 2, which serves as a negative feedback loop by suppressing activation of MEK1.Synonyms: Dual specificity mitogen-activated protein kinase kinase 1, ERK activator kinase 1, MAP kinase kinase 1, MAPK/ERK kinase 1, MAPKK 1, MEK1, PRKMK1

Gen-ID: 5604

NCBI Accession: NP_002746

UniProt: Q02750

Pathways: MAPK Signalweg, RTK Signalweg, Interferon-gamma Pathway, Fc-epsilon Rezeptor Signalübertragung, Neurotrophin Signalübertragung, Activation of Innate immune Response, Toll-Like Receptors Cascades, Autophagie, Signaling of Hepatocyte Growth Factor Receptor, BCR Signaling