TNFRSF8 Antikörper (APC)

Produktnummer ABIN192355

Dieser Maus Monoklonal Antikörper detektiert spezifisch TNFRSF8 in FACS. Es zeigt Reaktivität gegenüber Proben von Human.Es wurde in 1 Publikation zitiert.

Kurzübersicht für TNFRSF8 Antikörper (APC) (ABIN192355)

Target: TNFRSF8 (Tumor Necrosis Factor Receptor Superfamily, Member 8 (TNFRSF8))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser TNFRSF8 Antikörper ist konjugiert mit APC

Applikation: Flow Cytometry (FACS)

Klon: MEM-268

Produktdetails anti-TNFRSF8 Antikörper (APC)

Verwendungszweck: Anti-Hu CD30 APC

Kreuzreaktivität (Details): Human

Produktmerkmale: The antibody MEM-268 recognizes extracellular part of CD30 (Ki-1 antigen), a 105 kDa single chain glycoprotein expressed on Hodgkin's and Reed-Sternberg cells, it is also found in Burkitt's lymphomas, virus-infected T and B lymphocytes, and on normal B and T lymphocytes after activation (T lymphocytes that produce Th2-type cytokines and on CD4+/CD8+ T lymphocytes that co-express CD45RO and the IL4 receptor).

Aufreinigung: Purified antibody is conjugated with activated allophycocyanin (APC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

Immunogen: Expression vector containing CD30 cDNA (booster suspension of THP-1 cell line)

Isotyp: IgG2a, kappa

Anwendungsinformationen

Applikationshinweise: Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μL reagent / 100 μL of whole blood or 10⁶ cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Do not freeze,Protect from prolonged exposure to light

Lagerung: 4 °C

Informationen zur Lagerung: Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

Referenzen für anti-TNFRSF8 Antikörper (APC) (ABIN192355)

Pavlov, Martins, Delgado: "Development and validation of a fluorescent microsphere immunoassay for soluble CD30 testing." in: Clinical and vaccine immunology : CVI, Vol. 16, Issue 9, pp. 1327-31, (2009) (PubMed).

Details zu TNFRSF8

Target: TNFRSF8 (Tumor Necrosis Factor Receptor Superfamily, Member 8 (TNFRSF8))

Andere Bezeichnung: CD30

Hintergrund: TNF receptor superfamily member 8,CD30 is a type I transmembrane glycoprotein of the TNF receptor superfamily. CD30 was originally identified as a cell surface antigen of Hodgkins and Reed-Sternberg cells using monoclonal antibody Ki-1. The ligand for CD30 is CD30L (CD153). The binding of CD30 to CD30L mediates pleiotropic effects including cell proliferation, activation, differentiation, and apoptotic cell death. CD30 has a critical role in the pathophysiology of Hodgkin's disease and other CD30+ lymphomas. CD30 acts as a costimulatory molecule in thymic negative selection. In addition to its expression on Hodgkin's and Reed-Sternberg cells, CD30 is also found in some non-Hodgkin's lymphomas (including Burkitt's lymphomas), virus-infected T and B cells, and on normal T and B cells after activation. In T cells, CD30 expression is present on a subset of T cells that produce Th2-type cytokines and on CD4+/CD8+ thymocytes that co-express CD45RO and the IL4 receptor. Soluble form of CD30 (sCD30) serves as a marker reflecting Th2 immune response.,Ki-1, TNFRSF8, CD30L receptor, D1S166E

Gen-ID: 943

UniProt: P28908