MAPK6 Antikörper (AA 683-694)

Produktnummer ABIN1911983

Produktdetails anti-MAPK6 Antikörper

Target: MAPK6 (Mitogen-Activated Protein Kinase 6 (MAPK6))

Bindungsspezifität: AA 683-694

Reaktivität: Human, Maus, Rind (Kuh), Hund, Pferd, Schwein, Kaninchen, Huhn, Hamster

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser MAPK6 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Marke: IHC-plus™

Spezifität: Human MAPK6 / ERK3. Reported variants represent identical protein (NP_056621.4, NP_081694.1).

Aufreinigung: Immunoaffinity purified

Immunogen: Synthetic peptide C-HSPVGSPLKSIQ from an internal region (near C-terminus) of human MAPK6 / ERK3 (NP_002739.1). Percent identity by BLAST analysis: Hamster, Elephant, Panda, Dog, Bovine, Cat, Horse, Rabbit, Pig, Opossum, Turkey, Zebra finch, Chicken, Platypus, Lizard (100%), Rat, Xenopus (92%).

Type of Immunogen: Synthetic peptide

Anwendungsinformationen

Applikationshinweise: Approved: IHC, IHC-P (10 μg/mL), Peptide-ELISA (1:128000), WB (0.5 - 1 μg/mL)

Usage: Peptide ELISA: antibody detection limit dilution 1:128000. Western blot: In transfected HEK293 transiently expressing Mouse ERK3 / Mapk6 fused to GFP the band with expected size is intensified when co-expressed with MK5 as the stabilizer for ERK3. No bands are observed in transfected HEK293 with empty vector and the same band is observed using anti-GFP antibody. Data obtained from Dr. M. B. Menon, Inst. Biochemistry, Hannover Medical School, Germany. Recommended concentration, 0.5-1 μg/mL. Immunoprecipitation: MK5/ERK3 double knockout Mouse Embryonic Fibroblasts (MEFs) retrovirally transduced with MK5/ERK3 or empty vector (GFP) were lysed from confluent plates and used for IP with 1.5 μg EB11926. Western blots of the IP were labeled with rabbit anti-ERK3 serum (CST) or with mouse anti-MK5 (SC). An approx 100kD of ERK3 is only precipitated from lysates of those KO MEFs that have been rescued by the ERK3/MK5 expression construct as described in as described in PMID: 22508986. Data obtained from Dr. M. B. Menon, Inst. Biochemistry, Hannover Medical School, Germany.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Minimize freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Store at -20°C. Avoid freeze-thaw cycles.

Details zu MAPK6

Target: MAPK6 (Mitogen-Activated Protein Kinase 6 (MAPK6))

Andere Bezeichnung: MAPK6 / ERK3 (MAPK6 Produkte)

Synonyme: ERK3 antikoerper, HsT17250 antikoerper, PRKM6 antikoerper, p97MAPK antikoerper, 2610021I23Rik antikoerper, D130053K17Rik antikoerper, Erk3 antikoerper, Mapk4 antikoerper, Mapk63 antikoerper, Prkm4 antikoerper, Prkm6 antikoerper, ATMAPK6 antikoerper, ATMPK6 antikoerper, F18O19.10 antikoerper, MAP KINASE 6 antikoerper, MAP kinase 6 antikoerper, MAPK6 antikoerper, SUSERK3 antikoerper, erk3 antikoerper, p97mapk antikoerper, si:ch211-235f12.1 antikoerper, mitogen-activated protein kinase 6 antikoerper, MAP kinase 6 antikoerper, MAPK6 antikoerper, Mapk6 antikoerper, MPK6 antikoerper, mapk6 antikoerper

Hintergrund: Name/Gene ID: MAPK6
Subfamily: MAPK
Family: Protein Kinase

Synonyms: MAPK6, ERK-3, MAP kinase 6, MAPK 6, p97-MAPK, PRKM6, MAP kinase isoform p97, ERK3, HsT17250, p97MAPK

Gen-ID: 5597

NCBI Accession: NP_002739

Pathways: MAPK Signalweg, Neurotrophin Signalübertragung, Regulation of Muscle Cell Differentiation, Hepatitis C