GAD65 Antikörper (AA 4-22)

Produktnummer ABIN1897298

Produktdetails anti-GAD65 Antikörper

Target: GAD65 (GAD2) (Glutamate Decarboxylase 2 (Pancreatic Islets and Brain, 65kDa) (GAD2))

Bindungsspezifität: AA 4-22

Reaktivität: Human, Ratte, Affe

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser GAD65 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Radioimmunoassay (RIA)

Marke: IHC-plus™

Spezifität: Recognizes an epitope within the N-terminal (NT) region of Glutamate decarboxylase 2, otherwise known as GAD65/GAD2, an amphiphilic membrane-anchored protein and member of the group 2 decarboxylase family, principally expressed in the brain and also in pancreatic beta cells. GAD65 catalyzes the decarboxylation of glutamate to GABA, the major inhibitory neurotransmitter in the central nervous system. GAD65 is the 65kD isoform of GAD, encoded by the GAD2 gene, which is predominantly expressed by nerve termini, as oppose to the 67kD isoform (GAD67), which is predominantly found in the cell body, and is encoded by the GAD1 gene. Studies have shown the presence of autoantibodies to GAD65 (GAD65Ab) in autoimmune diseases, including Graves disease and Stiff Man Syndrome (SMS), but GAD65Ab are most prevalent in patients with Type I diabetes mellitus, and those at high risk of developing Type I diabetes. The N-Terminal region of GAD65 lacks the epitopes for GAD65Ab in Type I diabetes patients, and has been shown as essential for targeting the enzyme to GABA-containing secretory vesicles. Clone N-GAD65 has been shown to be highly specific for GAD65 and does not recognize GAD67.

Aufreinigung: Protein G purified

Immunogen: Keyhole Limpet Hemocyanin (KLH) conjugated synthetic peptide sequence PGSGFWSFGSEDGSGDSEN corresponding to amino acids 4-22 within the N-terminal region of human GAD65. Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan (100%), Gibbon, Galago, Rat, Ferret, Panda, Bovine, Cat, Dog, Horse, Pig (95%), Monkey, Marmoset, Mouse, Rabbit, Opossum, Guinea pig (89%).

Type of Immunogen: Synthetic peptide

Klon: N-GAD65

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Approved: IHC, IHC-P (5 μg/mL), IP, RIA, WB (1:100 - 1:1000)

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: PBS, 0.09 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: 4°C or -20°C, Avoid freeze-thaw cycles.

Details zu GAD65

Target: GAD65 (GAD2) (Glutamate Decarboxylase 2 (Pancreatic Islets and Brain, 65kDa) (GAD2))

Andere Bezeichnung: GAD65 (GAD2 Produkte)

Synonyme: GAD65 antikoerper, gad65 antikoerper, F12P19.12 antikoerper, F12P19_12 antikoerper, GLUTAMATE DECARBOXYLASE 2 antikoerper, glutamate decarboxylase 2 antikoerper, zgc:112198 antikoerper, 6330404F12Rik antikoerper, GAD(65) antikoerper, Gad-2 antikoerper, glutamate decarboxylase 2 antikoerper, glutamate decarboxylase 1 antikoerper, glutamic acid decarboxylase 2 antikoerper, GAD2 antikoerper, PTRG_06148 antikoerper, LOC9324534 antikoerper, Gad2 antikoerper, GAD1 antikoerper, gad2 antikoerper

Hintergrund: Name/Gene ID: GAD2

Synonyms: GAD2, GAD-65, Glutamate decarboxylase 2, GAD65

Gen-ID: 2572