JNK2 Antikörper (C-Term)

Produktnummer ABIN1882172

Der Kaninchen Polyklonal Anti-JNK2-Antikörper wurde für WB und IHC (p) validiert. Er ist geeignet, JNK2 in Proben von Human zu detektieren. Es sind 4+ Publikationen verfügbar.

Kurzübersicht für JNK2 Antikörper (C-Term) (ABIN1882172)

Target: JNK2 (MAPK9) (Mitogen-Activated Protein Kinase 9 (MAPK9))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser JNK2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Klon: RB2510

Produktdetails anti-JNK2 Antikörper

Bindungsspezifität: AA 349-379, C-Term

Aufreinigung: This antibody is purified through a protein A column, followed by peptide affinity purification.

Immunogen: This JNK2 (MAPK9) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 349-379 amino acids from the C-terminal region of human JNK2 (MAPK9).

Isotyp: Ig Fraction

Anwendungsinformationen

Applikationshinweise: WB: 1:1000. IHC-P: 1:50~100

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Haltbarkeit: 6 months

Referenzen für anti-JNK2 Antikörper (ABIN1882172)

Tetsunaga, Nishida, Furumatsu, Naruse, Hirohata, Yoshida, Saito, Ozaki: "Regulation of mechanical stress-induced MMP-13 and ADAMTS-5 expression by RUNX-2 transcriptional factor in SW1353 chondrocyte-like cells." in: Osteoarthritis and cartilage / OARS, Osteoarthritis Research Society, Vol. 19, Issue 2, pp. 222-32, (2011) (PubMed).

Gupta, Barrett, Whitmarsh, Cavanagh, Sluss, Dérijard, Davis: "Selective interaction of JNK protein kinase isoforms with transcription factors." in: The EMBO journal, Vol. 15, Issue 11, pp. 2760-70, (1996) (PubMed).

Kallunki, Su, Tsigelny, Sluss, Dérijard, Moore, Davis, Karin: "JNK2 contains a specificity-determining region responsible for efficient c-Jun binding and phosphorylation." in: Genes & development, Vol. 8, Issue 24, pp. 2996-3007, (1995) (PubMed).

Sluss, Barrett, Dérijard, Davis: "Signal transduction by tumor necrosis factor mediated by JNK protein kinases." in: Molecular and cellular biology, Vol. 14, Issue 12, pp. 8376-84, (1994) (PubMed).

Details zu JNK2

Target: JNK2 (MAPK9) (Mitogen-Activated Protein Kinase 9 (MAPK9))

Andere Bezeichnung: JNK2 (MAPK9)

Hintergrund: JNK2 responds to activation by environmental stress and pro-inflammatory cytokines by phosphorylating a number of transcription factors, primarily components of AP-1 such as c-Jun and ATF2 and thus regulates AP-1 transcriptional activity. In T-cells, JNK1 and JNK2 are required for polarized differentiation of T-helper cells into Th1 cells. JNK2 isoforms display different binding patterns: alpha-1 and alpha-2 preferentially bind to c-Jun, whereas beta-1 and beta-2 bind to ATF2. However, there is no correlation between binding and phosphorylation, which is achieved at about the same efficiency by all isoforms. JUNB is not a substrate for JNK2 alpha-2, and JUND binds only weakly to it. JNK2 is activated by threonine and tyrosine phosphorylation by either of two dual specificity kinases, MAP2K4 and MAP2K7. It is inhibited by dual specificity phosphatases, such as DUSP1. The protein has been shown to bind to at least three scaffolding proteins, MAPK8IP1/JIP-1, MAPK8IP2/JIP-2 and MAPK8IP3/JIP-3/JSAP1. These proteins also bind other components of the JNK signaling pathway

Molekulargewicht: 48139

NCBI Accession: NP_001128516, NP_002743, NP_620707, NP_620708, NP_620709

UniProt: P45984

Pathways: MAPK Signalweg, WNT Signalweg, TLR Signalweg, Fc-epsilon Rezeptor Signalübertragung, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Endopeptidase Activity, Hepatitis C, Toll-Like Receptors Cascades, BCR Signaling, S100 Proteine