This antibody is purified through a protein A column, followed by peptide affinity purification.
Immunogen
This PSMD5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 428-457 amino acids from the C-terminal region of human PSMD5.
PSMD5
Reaktivität: Human
WB, ELISA, IHC
Wirt: Kaninchen
Polyclonal
unconjugated
Applikationshinweise
WB: 1:1000
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Liquid
Buffer
Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.
Konservierungsmittel
Sodium azide
Vorsichtsmaßnahmen
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Lagerung
4 °C,-20 °C
Haltbarkeit
6 months
Roelofs, Park, Haas, Tian, McAllister, Huo, Lee, Zhang, Shi, Gygi, Finley: "Chaperone-mediated pathway of proteasome regulatory particle assembly." in: Nature, Vol. 459, Issue 7248, pp. 861-5, (2009) (PubMed).
Kaneko, Hamazaki, Iemura, Sasaki, Furuyama, Natsume, Tanaka, Murata: "Assembly pathway of the Mammalian proteasome base subcomplex is mediated by multiple specific chaperones." in: Cell, Vol. 137, Issue 5, pp. 914-25, (2009) (PubMed).
Listovsky, Oren, Yudkovsky, Mahbubani, Weiss, Lebendiker, Brandeis: "Mammalian Cdh1/Fzr mediates its own degradation." in: The EMBO journal, Vol. 23, Issue 7, pp. 1619-26, (2004) (PubMed).
The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits, 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a non-ATPase subunit of the 19S regulator base.