PRKAA1 Antikörper (pSer487)

Produktnummer ABIN1842361

Produktdetails anti-PRKAA1 Antikörper

Target: PRKAA1 (Protein Kinase, AMP-Activated, alpha 1 Catalytic Subunit (PRKAA1))

Bindungsspezifität: pSer487

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser PRKAA1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Spezifität: Rabbit Anti-AMPKalpha,1 (Phospho-Ser487) Polyclonal Antibody detects endogenous levels of AMPKalpha,1 only when phosphorylated at serine 487.

Produktmerkmale: Molecular Weight: Predicted Band Size 63 kDa, Observed Band Size 63 kDa

Aufreinigung: Rabbit Anti-AMPKalpha,1 (Phospho-Ser487) Polyclonal Antibody is affinity-purified from rabbit antiserum by affinity chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide is removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

Immunogen: Synthesized phosphopeptide derived from human AMPKalpha,1 around pSer487 (S-G-SP-V-S)

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blot: 1:500-1:1,000,
IHC: 1:50-1:100,
IF: 1:100-1:200

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1 mg/mL

Buffer: phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide, and 50 % glycerol

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Handhabung: Briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container's cap because small volumes of antibody will occasionally become entrapped in the seal of the product vial during shipment and storage.
Avoid repeated freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Store at -20 °C for 1 year.

Details zu PRKAA1

Target: PRKAA1 (Protein Kinase, AMP-Activated, alpha 1 Catalytic Subunit (PRKAA1))

Andere Bezeichnung: AMPKalpha1 (PRKAA1 Produkte)

Synonyme: AMPK antikoerper, AMPKa1 antikoerper, cb116 antikoerper, im:7154392 antikoerper, sb:cb130 antikoerper, wu:fa94c10 antikoerper, AMPK1 antikoerper, AMPKalpha1 antikoerper, AI194361 antikoerper, AI450832 antikoerper, AL024255 antikoerper, C130083N04Rik antikoerper, ampk antikoerper, ampka1 antikoerper, PRKAA1 antikoerper, protein kinase AMP-activated catalytic subunit alpha 1 antikoerper, protein kinase, AMP-activated, alpha 1 catalytic subunit antikoerper, protein kinase, AMP-activated, alpha 1 catalytic subunit L homeolog antikoerper, PRKAA1 antikoerper, prkaa1 antikoerper, Prkaa1 antikoerper, prkaa1.L antikoerper

Hintergrund: Responsible for the regulation of fatty acid synthesis by phosphorylation of acetyl-COA carboxylase. It also regulates cholesterol synthesis via phosphorylation and inactivation of hormone-sensitive lipase and hydroxymethylglutaryl-COA reductase. Appears to act as a metabolic stress-sensing protein kinase switching off biosynthetic pathways when cellular ATP levels are depleted and when 5AMP rises in response to fuel limitation and/or hypoxia. This is a catalytic subunit.

Pathways: AMPK Signaling, Carbohydrate Homeostasis, Regulation of Carbohydrate Metabolic Process, Warburg Effekt