His Tag Antikörper (Biotin)

Details zu Produkt Nr. ABIN1842272, Anbieter: Anmelden zum Anzeigen
Antigen
Reaktivität
Tag
604
26
15
6
5
2
1
Wirt
Kaninchen
427
185
24
23
8
8
3
2
Klonalität
Polyklonal
Konjugat
Biotin
58
46
36
16
13
12
10
10
9
9
9
9
8
8
8
7
7
7
5
4
4
4
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Applikation
Western Blotting (WB)
575
406
164
107
88
83
80
27
12
11
9
7
7
6
6
6
5
4
3
3
2
2
1
1
1
1
1
1
1
1
1
1
Optionen
Hersteller
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Immunogen His epitope tag peptide HHHHHH conjugated to KLH.
Sequenz HHHHHH
Isotyp IgG
Spezifität His-tag antibody [Biotin], pAb, Rabbit specifically reacts with fusion proteins containing His tagged fusion proteins.
Produktmerkmale His-tag antibody [Biotin], pAb, Rabbit is the biotin conjugated Rabbit anti His antibody. It is suitable for detecting the expression level of His tagged proteins. His-tag antibody [Biotin], pAb, Rabbit is the biotin conjugated Rabbit anti His antibody. The antibody recognizes fusion protein with His tag which is located at either C-terminal, or internal regions of the protein. It is suitable for detecting the expression level of His tagged proteins.
Reinigung Immunoaffinity chromatography
Substanzklasse Tag
Applikations-hinweise Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.

Western blot: 0. 2-1 µg/mL
Beschränkungen Nur für Forschungszwecke einsetzbar
Format Lyophilized
Rekonstitution Reconstitute the lyophilized antibody with deionized water (or equivalent) to a final antibody concentration of 0.5 mg/mL.
Konzentration 0.5 mg/mL
Buffer lyophilized with PBS, pH 7.4, containing 1 % BSA and 0.02 % sodium azide
Konservierungs-mittel Sodium azide
Vorsichtsmaßnahmen WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handhabung Avoid repeated freezing and thawing.
Lagerung -20 °C
Informationen zur Lagerung The antibody is stable in lyophilized form if stored at -20 °C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8 °C. For long term storage, aliquot and store at -20 °C or below.
Produkt verwendet in: Dong, Blissard: "Functional analysis of the Autographa californica multiple nucleopolyhedrovirus GP64 terminal fusion loops and interactions with membranes." in: Journal of virology, Vol. 86, Issue 18, pp. 9617-28, 2012 (PubMed).

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