Dieser His Tag Antikörper ist konjugiert mit Biotin
Applikation
Western Blotting (WB)
Sequenz
HHHHHH
Spezifität
His-tag antibody [Biotin], pAb, Rabbit specifically reacts with fusion proteins containing His tagged fusion proteins.
Produktmerkmale
His-tag antibody [Biotin], pAb, Rabbit is the biotin conjugated Rabbit anti His antibody. It is suitable for detecting the expression level of His tagged proteins. His-tag antibody [Biotin], pAb, Rabbit is the biotin conjugated Rabbit anti His antibody. The antibody recognizes fusion protein with His tag which is located at either C-terminal, or internal regions of the protein. It is suitable for detecting the expression level of His tagged proteins.
WB, ELISA, IP
Wirt: Maus
Monoclonal
33D10-D2
Biotin
Applikationshinweise
Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
Western blot: 0. 2-1 µg/mL
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Lyophilized
Rekonstitution
Reconstitute the lyophilized antibody with deionized water (or equivalent) to a final antibody concentration of 0.5 mg/mL.
Konzentration
0.5 mg/mL
Buffer
lyophilized with PBS, pH 7.4, containing 1 % BSA and 0.02 % sodium azide
Konservierungsmittel
Sodium azide
Vorsichtsmaßnahmen
WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handhabung
Avoid repeated freezing and thawing.
Lagerung
-20 °C
Informationen zur Lagerung
The antibody is stable in lyophilized form if stored at -20 °C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8 °C. For long term storage, aliquot and store at -20 °C or below.
Dong, Blissard: "Functional analysis of the Autographa californica multiple nucleopolyhedrovirus GP64 terminal fusion loops and interactions with membranes." in: Journal of virology, Vol. 86, Issue 18, pp. 9617-28, (2012) (PubMed).