WNT3A
Reaktivität: Human
WB
Wirt: Kaninchen
Polyclonal
unconjugated
Applikationshinweise
ELISA: Indirect: To detect hWNT-3a by indirect ELISA (using 100 μL/well antibody solution) aconcentration of 0.5 - 2.0 μg/mL is required. In conjunction with compatible secondaryreagents, it allows the detection of at least 0.2 - 0.4 ng/well of recombinant hWNT-3a. Sandwich: To detect hWNT-3a by sandwich ELISA (using 100 μL/well antibody solution) aconcentration of 0.5 - 2.0 μg/mL is required. In conjunction with Biotinylated Anti-HumanWNT-3a as a detection antibody, it allows the detection of at least 0.2 - 0.4 ng/well ofrecombinant hWNT-3a. Western blot: 0.1 - 0.2 μg/mL (reducing and non-reducing conditions). Neutralisation. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Rekonstitution
Restore in sterile water to a concentration of 0.1-1.0 mg/mL.
Buffer
PBS, pH 7.2
Handhabung
Avoid repeated freezing and thawing.
Lagerung
-20 °C
Informationen zur Lagerung
Store the lyophilized antibody at -20 °C. Following reconstitution it is stable for two weeks at 2-8 °C. Frozen aliquots are stable for 6 months when stored at -20 °C.
Haltbarkeit
6 months
Target
WNT3A
(Wingless-Type MMTV Integration Site Family, Member 3A (WNT3A))