ARC (AA 1-214) Antikörper

Produktnummer ABIN1742333

Produktdetails

Target: ARC

Bindungsspezifität: AA 1-214

Reaktivität: Maus, Ratte

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Unkonjugiert

Applikation: ELISA, Immunoprecipitation (IP)

Spezifität: Specific for Arc.

Aufreinigung: purified IgG. Azide was added before lyophilization.

Immunogen: Strep-Tag® fusion protein of full-length mouse arc. Epitope Binds within first 214 aa.

Klon: 48B2

Isotyp: IgG2b

Anwendungsinformationen

Applikationshinweise: WB: not recommended
ICC: not tested yet
IHC: not tested yet

Kommentare:

ELISA: Suitable as capture antibody for sandwich-ELISA as detector antibody (protocol for sandwich-ELISA).

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: For reconstitution add 100 µL H2O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20 °C until use.

Buffer: PBS, 0.02% sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Do not store diluted antibody solutions unless you add detergent or carrier proteins such as goat serum, BSA or others. IgG sticks to glass and plastic. Any IgG solution below 0.1 mg/mL protein will quickly adsorb and denature and thus loose activity! Repetitive freeze-thawing of dilute purified IgG is almost certain to lead to substantial losses.

Lagerung: -20 °C

Informationen zur Lagerung: Unlabeled antibodies are stable in this form without loss of quality at ambient temperatures for several weeks or even months. They can be stored at 4 °C for several years.

Referenzen

Izumi, Ishimoto, Yamamoto, Mori: "Application of hairless mouse strain to bioluminescence imaging of Arc expression in mouse brain." in: BMC neuroscience, Vol. 18, Issue 1, pp. 18, (2017) (PubMed).

Gerace, Landucci, Totti, Bani, Guasti, Baronti, Moroni, Mannaioni, Pellegrini-Giampietro: "Ethanol Toxicity During Brain Development: Alterations of Excitatory Synaptic Transmission in Immature Organotypic Hippocampal Slice Cultures." in: Alcoholism, clinical and experimental research, Vol. 40, Issue 4, pp. 706-16, (2016) (PubMed).

Lee, Kang, Kim, Yoon, Kim, Moon: "Enhanced expression of immediate-early genes in mouse hippocampus after trimethyltin treatment." in: Acta histochemica, (2016) (PubMed).

Alvarez-Dieppa, Griffin, Cavalier, McIntyre: "Vagus Nerve Stimulation Enhances Extinction of Conditioned Fear in Rats and Modulates Arc Protein, CaMKII, and GluN2B-Containing NMDA Receptors in the Basolateral Amygdala." in: Neural plasticity, Vol. 2016, pp. 4273280, (2016) (PubMed).

Vasileva, Horstmann, Geumann, Gitler, Kuner: "Synapsin-dependent reserve pool of synaptic vesicles supports replenishment of the readily releasable pool under intense synaptic transmission." in: The European journal of neuroscience, Vol. 36, Issue 8, pp. 3005-20, (2012) (PubMed).

Antigendetails

Target: ARC

Synonyme: activity regulated cytoskeleton associated protein antikoerper, Arc antikoerper

Hintergrund: Synonyms: Arg 3.1