BSA Antikörper (Biotin)

Produktnummer ABIN1607854

Produktdetails anti-BSA Antikörper (Biotin)

Target: BSA (Bovine Serum Albumin (BSA))

Reaktivität: Rind (Kuh)

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser BSA Antikörper ist konjugiert mit Biotin

Applikation: ELISA, Western Blotting (WB), Immunohistochemistry (IHC)

Kreuzreaktivität (Details): Cross reactivity against Albumin from other sources may occur but have not been specifically determined.

Aufreinigung: Bovine serum antibody was purified as an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Rabbit Serum as well as purified and partially purified Albumin (Bovine Serum].

Endotoxin-Niveau: Low Endotoxin : No

Immunogen: Albumin (Bovine Serum)
Immunogen Type: Native Protein

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Anti-BSA antibody is suitable for detection of bovine serum albumin in biological samples by ELISA, western blot and immunohistochemisty assays. Specific dilutions are recommended but should be opitmized by researcher.
ELISA Dilution: 1:50.000
Immunohistochemistry Dilution: 1:300 - 1:2.500
Western Blot Dilution: 1:1.000 - 1:5.000

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 10 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Polyethylene Glycol (PEG-8000)

Handhabung: Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Store vial at -20 °C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below.

Haltbarkeit: Expiration date is one (1) year from date of opening.

Details zu BSA

Target: BSA (Bovine Serum Albumin (BSA))

Abstract: BSA Produkte

Synonyme: PRO0883 antikoerper, PRO0903 antikoerper, PRO1341 antikoerper, albumin antikoerper, ALB antikoerper

Hintergrund: Anti-BSA antibody is specific for the full-length BSA precursor protein is 607 amino acids in length. An N-terminal 18-residue signal peptide is cut off from the precursor protein upon secretion, and an additional 4 amino acids is cleaved to yield the mature BSA protein that contains 585 amino acids. BSA has numerous biochemical applications including ELISAs (Enzyme-Linked Immunosorbent Assay), immunoblots, and immunohistochemistry, and it is also used as a nutrient in cell and microbial culture. BSA is used as a stabilizer for some proteins and enzymes. This protein does not affect other enzymes that do not need it for stabilization. Synonyms: ALB antibody, Albumin antibody, Allergen Bos d 6 antibody, BSA antibody, DKFZp779N1935 antibody

Gen-ID: 280717

UniProt: P02769

Pathways: Lipid Metabolism