Aspartate Transaminase Antikörper (Biotin)

Produktnummer ABIN1607837

Produktdetails anti-Aspartate Transaminase Antikörper (Biotin)

Target: Aspartate Transaminase (Ast)

Reaktivität: Schwein

Wirt: Schaf

Klonalität: Polyklonal

Konjugat: Dieser Aspartate Transaminase Antikörper ist konjugiert mit Biotin

Applikation: ELISA, Western Blotting (WB)

Kreuzreaktivität (Details): Cross reactivity against Aspartate Aminotransferase (AST) from other sources may occur but has not been specifically determined.

Reinheit: Anti-Aspartate Transaminase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Sheep Serum as well as purified and partially purified Aspartate Aminotransferase (AST) [Pig Heart].

Endotoxin-Niveau: Low Endotoxin : No

Immunogen: Aspartate Aminotransferase (AST) [Pig Heart]
Immunogen Type: Native Protein

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Anti-Aspartate Transaminase has been assayed against 1.0 µg of Aspartate Aminotransferase (AST in a standard capture ELISA using Peroxidase Conjugated Streptavidin #S000-03 and ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:4.000 to 1:20.000 of the reconstitution concentration is suggested for this product. This product has been assayed against 1.0 µg of Aspartate Aminotransferase (AST in a standard capture ELISA using Peroxidase Conjugated Streptavidin #S000-03 and ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:4.000 to 1:20.000 of the reconstitution concentration is suggested for this product.
ELISA Dilution: 1:5.000 - 1:25.000
Western Blot Dilution: 1:500 - 1:3.000

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 10 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Handhabung: Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Store vial at -20 °C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below.

Haltbarkeit: Expiration date is one (1) year from date of opening.

Details zu Aspartate Transaminase

Target: Aspartate Transaminase (Ast)

Andere Bezeichnung: Aspartate Transaminase (Ast Produkte)

Synonyme: Afu6g02490 antikoerper, Afu2g09650 antikoerper, aspartate transaminase antikoerper, Aspartate transaminase antikoerper, Aspartate transaminase, putative antikoerper, aspB antikoerper, CNM00360 antikoerper, AFUA_6G02490 antikoerper, AFUA_2G09650 antikoerper, AAT_1 antikoerper, Dd586_1627 antikoerper, aspB3 antikoerper, Fbal_1917 antikoerper, Emin_0749 antikoerper, CGB_M0340W antikoerper, Sgly_0435 antikoerper, Weevi_1216 antikoerper, Selsp_1595 antikoerper, Trebr_2143 antikoerper

Hintergrund: Anti-Aspartate Transaminase antibody is present in the cytoplasm of cells, belonging to the class-I pyridoxal-phosphate-dependent aminotransferase family. Playing an active role in amino acid metabolism, Anti-Aspartate Transaminase antibody is ideal for investigators interested in Metabolism, Cancer, and Signal Transduction research. Synonyms: Aspartate aminotransferase 1 antibody, Aspartate aminotransferase cytoplasmic antibody, Aspartate aminotransferase cytosolic antibody, ec 2.6.1.1 antibody, GIG 18 antibody, GIG18 antibody, Glutamate oxaloacetate transaminase 1 antibody

Gen-ID: 396967

UniProt: P00503