Aspartate Transaminase Antikörper (HRP)
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- Target Alle Aspartate Transaminase (Ast) Produkte
- Aspartate Transaminase (Ast)
- Reaktivität
- Schwein
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Wirt
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Schaf
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Klonalität
- Polyklonal
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Konjugat
- Dieser Aspartate Transaminase Antikörper ist konjugiert mit HRP
- Applikation
- ELISA, Western Blotting (WB)
- Kreuzreaktivität (Details)
- Cross reactivity against Aspartate Aminotransferase (AST) from other sources may occur but has not been specifically determined.
- Aufreinigung
- Aspartate Transaminase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Sheep Serum as well as purified and partially purified Aspartate Aminotransferase (AST) [Pig Heart].
- Immunogen
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Immunogen: Aspartate Aminotransferase (AST) [Pig Heart]
Immunogen Type: Native Protein
- Isotyp
- IgG
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- Applikationshinweise
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Application Note: Anti-Aspartate Transaminase has been assayed against 1.0 μg of Aspartate Aminotransferase (AST) [Pig Heart] in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:20,000 to 1:100,000 of the reconstitution concentration is suggested for this product.
Western Blot Dilution: 1:1,000 - 1:5,000
ELISA Dilution: 1:10,000 - 1:40,000
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Format
- Lyophilized
- Rekonstitution
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Reconstitution Volume: 100 μL
Reconstitution Buffer: Restore with deionized water (or equivalent)
- Konzentration
- 1 mg/mL
- Buffer
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
- Konservierungsmittel
- Gentamicin sulfate
- Vorsichtsmaßnahmen
- This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Lagerung
- 4 °C,-20 °C
- Informationen zur Lagerung
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
- Haltbarkeit
- 12 months
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- Target
- Aspartate Transaminase (Ast)
- Andere Bezeichnung
- Aspartate Transaminase (Ast Produkte)
- Synonyme
- Afu6g02490 antikoerper, Afu2g09650 antikoerper, aspartate transaminase antikoerper, Aspartate transaminase antikoerper, Aspartate transaminase, putative antikoerper, aspB antikoerper, CNM00360 antikoerper, AFUA_6G02490 antikoerper, AFUA_2G09650 antikoerper, AAT_1 antikoerper, Dd586_1627 antikoerper, aspB3 antikoerper, Fbal_1917 antikoerper, Emin_0749 antikoerper, CGB_M0340W antikoerper, Sgly_0435 antikoerper, Weevi_1216 antikoerper, Selsp_1595 antikoerper, Trebr_2143 antikoerper
- Hintergrund
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Synonyms: Aspartate aminotransferase 1 antibody, Aspartate aminotransferase cytoplasmic antibody, Aspartate aminotransferase cytosolic antibody, ec 2.6.1.1 antibody, GIG 18 antibody, GIG18 antibody, Glutamate oxaloacetate transaminase 1 antibody
Background: Aspartate Transaminase is an important regulator of levels of glutamate, the major excitatory neurotransmitter of the vertebrate central nervous system. It acts as a scavenger of glutamate in brain neuroprotection. The aspartate aminotransferase activity is involved in hepatic glucose synthesis during development and in adipocyte glyceroneogenesis. Aspartate Transaminase is found in the liver, heart, skeletal muscle, kidneys, brain, and red blood cells, and it is commonly measured clinically as a marker for liver health.
Gene Name: GOT1
- Gen-ID
- 396967
- UniProt
- P00503
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