E-cadherin Antikörper (AA 650-700)

Produktnummer ABIN1574190

Produktdetails anti-E-cadherin Antikörper

Target: E-cadherin (CDH1) (Cadherin 1, Type 1, E-Cadherin (Epithelial) (CDH1))

Bindungsspezifität: AA 650-700

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser E-cadherin Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Flow Cytometry (FACS)

Spezifität: E-Cadherin Antibody detects human and mouse endogenous levels of E-Cadherin. It is predicted to react with rat E-Cadherin protein according to sequence homology.Positive Control: HepG2 and NIH/3T3

Kreuzreaktivität (Details): E-Cadherin Antibody detects human and mouse endogenous levels of E-Cadherin. It is predicted to react with rat E-Cadherin protein according to sequence homology.Positive Control: HepG2 and NIH/3T3

Aufreinigung: Immunoaffinity chromatography

Immunogen: KLH-coupled synthetic peptide within AA 650–700 of human E-Cadherin

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
Western blot: 0.5-1 µg/mLFlow cytometry: 1-3 µg for 1 x 106 cellsOther Applications: user-optimized

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Buffer: PBS, pH 7.4, containing 0.02 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.

Referenzen für anti-E-cadherin Antikörper (ABIN1574190)

Dong, Kaneuchi, Xiong, Cao, Cai, Liu, Guo, Ju, Jia, Konno, Watari, Hosaka, Sudo, Sakuragi: "Identification of KLF17 as a novel epithelial to mesenchymal transition inducer via direct activation of TWIST1 in endometrioid endometrial cancer." in: Carcinogenesis, Vol. 35, Issue 4, pp. 760-8, (2014) (PubMed).

Konno, Dong, Xiong, Suzuki, Lu, Cai, Watari, Mitamura, Hosaka, Hanley, Kudo, Sakuragi: "MicroRNA-101 targets EZH2, MCL-1 and FOS to suppress proliferation, invasion and stem cell-like phenotype of aggressive endometrial cancer cells." in: Oncotarget, Vol. 5, Issue 15, pp. 6049-62, (2014) (PubMed).

Erin, Kale, Tanr?över, Köksoy, Duymu?, Korcum: "Differential characteristics of heart, liver, and brain metastatic subsets of murine breast carcinoma." in: Breast cancer research and treatment, Vol. 139, Issue 3, pp. 677-89, (2013) (PubMed).

Details zu E-cadherin

Target: E-cadherin (CDH1) (Cadherin 1, Type 1, E-Cadherin (Epithelial) (CDH1))

Andere Bezeichnung: E-Cadherin (CDH1 Produkte)

Synonyme: Arc-1 antikoerper, CD324 antikoerper, CDHE antikoerper, ECAD antikoerper, LCAM antikoerper, UVO antikoerper, E-cadherin antikoerper, cdh1 antikoerper, si:dz180o5.2 antikoerper, uvo antikoerper, lcam antikoerper, E-Cad antikoerper, XBcad antikoerper, l-cam antikoerper, xcdh1 antikoerper, cdhc-A antikoerper, xb-cad antikoerper, XTCAD-1 antikoerper, c-cadherin antikoerper, cadherin-1 antikoerper, uvomorulin antikoerper, XB-cadherin antikoerper, arc-1 antikoerper, cd324 antikoerper, cdhe antikoerper, ecad antikoerper, AA960649 antikoerper, E-cad antikoerper, Ecad antikoerper, L-CAM antikoerper, Um antikoerper, cadherin 1 antikoerper, si:busm1-180o5.2 antikoerper, cadherin 1, type 1, E-cadherin (epithelial) antikoerper, cadherin 1, type 1 S homeolog antikoerper, CDH1 antikoerper, si:busm1-180o5.2 antikoerper, cdh1 antikoerper, Cdh1 antikoerper, cdh1.S antikoerper

Hintergrund: E-Cadherin is a key cell adhesion molecule located at adherens junctions (AJs) and undergoes endocytosis when AJs are disrupted by the action of extracellular signals. It is a member of cadherin family that includes cadhesin repeats of approximately 100 residues in their extracellular domian. E-Cadherin plays a crucial role in the maintenance of the epithelial junctional complex and is an important molecule in maintaining epithelial integrity. The expression of E-Cadherin might be associated with the invasive potential of epithelial neoplasms. Defects in E-Cadherin are a cause of gastric cancer, also known as hereditary familial diffuse gastric cancer (HDGC).E-Cadherin Antibody is developed in rabbit using a KLH-coupled synthetic peptide within residues 650-00 of human E-Cadherin (Swiss Prot: P12830).

Pathways: WNT Signalweg, Sensory Perception of Sound, Cell-Cell Junction Organization, Tube Formation