Vimentin Antikörper (AA 400-500)

Produktnummer ABIN1574034

Produktdetails anti-Vimentin Antikörper

Target: Vimentin (VIM)

Bindungsspezifität: AA 400-500

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Vimentin Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Flow Cytometry (FACS)

Spezifität: Vimentin Antibody, pAb, Rabbit detects endogenous levels of human vimentin protein. Sequence homology predicts that it will also react with mouse and rat vimentin.

Kreuzreaktivität (Details): Vimentin Antibody, pAb, Rabbit detects endogenous levels of human vimentin protein. Sequence homology predicts that it will also react with mouse and rat vimentin.

Aufreinigung: Immunoaffinity chromatography

Immunogen: Synthetic peptide (KLH-coupled) derived from within AA 400-500 of human vimentin

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.

ELISA: 0.05-0.2 µg/mL
Western blot: 0.5-1 µg/mLImmunohistochemistry: 5-10 µg/mLFlow cytometry: 1-3 µg for 1 x 10⁶ cells
Other applications: user-optimized

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Buffer: PBS, pH 7.4, containing 0.02 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.

Details zu Vimentin

Target: Vimentin (VIM)

Andere Bezeichnung: Vimentin (VIM Produkte)

Synonyme: CTRCT30 antikoerper, cb28 antikoerper, vime antikoerper, vim antikoerper, vim1 antikoerper, vim2 antikoerper, VIM antikoerper, Vimentin antikoerper, vim4 antikoerper, vimentin antikoerper, vimentin L homeolog antikoerper, vimentin S homeolog antikoerper, VIM antikoerper, Vim antikoerper, vim antikoerper, vim.L antikoerper, vim.S antikoerper

Hintergrund: Vimentin is a member of the intermediate filament protein family. Intermediate filaments are an important structural feature of eukaryotic cells. They, along with microtubules and actin microfilaments, make up the cytoskeleton. Despite the fact that most intermediate filaments exist as stable structures, in fibroblasts, vimentin take on a dynamic structure.Vimentin Antibody, pAb, Rabbit is developed in rabbit using a KLH-coupled synthetic peptide within residues 400-500 of human vimentin protein (Swiss Prot: P08670).

Pathways: Caspase Kaskade in der Apoptose