Vimentin Antikörper

Produktnummer ABIN6940879

Dieser Maus Monoklonal Antikörper detektiert spezifisch Vimentin in WB, IHC und FACS. Es zeigt Reaktivität gegenüber Proben von Human, Schwein, Rind (Kuh), Huhn, Hund, Cat und Ziege.

Kurzübersicht für Vimentin Antikörper (ABIN6940879)

Target: Vimentin (VIM)

Reaktivität: Human, Schwein, Rind (Kuh), Huhn, Hund, Cat, Ziege

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Vimentin Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS)

Klon: VM452

Produktdetails anti-Vimentin Antikörper

Verwendungszweck: Vimentin (Mesenchymal Cell Marker) Antibody

Spezifität: This MAb reacts with a 58 kDa protein identified as vimentin. It shows no cross-reaction with other closely related intermediate filament proteins (IFP's) such as desmin, keratin, neurofilament, and glial fibrillary acid protein.Anti-vimentin alone is of limited value as a diagnostic tool, however, when used in panels with other antibodies, it is useful for the sub-classification of a given tumor. Expression of vimentin, when used in conjunction with anti-keratin, is helpful when distinguishing melanomas from undifferentiated carcinomas and large cell lymphomas. All melanomas and Schwannomas react strongly with anti-vimentin. It labels a variety of mesenchymal cells, including melanocytes, lymphocytes, endothelial cells, and fibroblasts. Non-reactivity of anti-vimentin is often considered more useful than its positive reactivity, since there are a few tumors that do not contain vimentin, e.g. hepatoma and seminoma. Anti-vimentin is also useful as a tissue process control reagent.

Keine Kreuzreaktivität: Maus, Ratte (Rattus)

Aufreinigung: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Recombinant full-length human vimentin protein

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise:

Positive Control: Raji, Jurkat or HeLa cells. Sarcomas or Melanomas.

Known Application: Flow Cytometry (1-2 μg/million cells), Western Blot (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (0.5-1.0 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 200 μg/mL

Buffer: Prepared in 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Haltbarkeit: 24 months

Details zu Vimentin

Target: Vimentin (VIM)

Andere Bezeichnung: VIM

Hintergrund:

VIM

Raji, A375 or Jurkat cells. Human melanomas.

Molekulargewicht: 57-60kDa

Gen-ID: 7431, 455493

UniProt: P08670

Pathways: Caspase Kaskade in der Apoptose