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Vimentin Antikörper

VIM Reaktivität: Human, Schwein, Rind (Kuh), Huhn, Hund, Cat, Ziege WB, IHC, FACS, StM Wirt: Maus Monoclonal VM452 unconjugated
Produktnummer ABIN6940879
  • Target Alle Vimentin (VIM) Antikörper anzeigen
    Vimentin (VIM)
    Reaktivität
    • 333
    • 127
    • 126
    • 60
    • 58
    • 44
    • 42
    • 36
    • 30
    • 21
    • 19
    • 14
    • 13
    • 8
    • 7
    • 6
    • 6
    • 4
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Human, Schwein, Rind (Kuh), Huhn, Hund, Cat, Ziege
    Wirt
    • 190
    • 129
    • 9
    • 6
    • 4
    • 4
    • 2
    Maus
    Klonalität
    • 198
    • 145
    Monoklonal
    Konjugat
    • 200
    • 19
    • 13
    • 13
    • 11
    • 7
    • 7
    • 7
    • 7
    • 7
    • 7
    • 7
    • 4
    • 4
    • 4
    • 4
    • 4
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    Dieser Vimentin Antikörper ist unkonjugiert
    Applikation
    • 282
    • 131
    • 100
    • 98
    • 96
    • 65
    • 49
    • 40
    • 39
    • 28
    • 23
    • 20
    • 8
    • 5
    • 4
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Staining Methods (StM)
    Spezifität
    This MAb reacts with a 58 kDa protein identified as vimentin. It shows no cross-reaction with other closely related intermediate filament proteins (IFP's) such as desmin, keratin, neurofilament, and glial fibrillary acid protein.Anti-vimentin alone is of limited value as a diagnostic tool, however, when used in panels with other antibodies, it is useful for the sub-classification of a given tumor. Expression of vimentin, when used in conjunction with anti-keratin, is helpful when distinguishing melanomas from undifferentiated carcinomas and large cell lymphomas. All melanomas and Schwannomas react strongly with anti-vimentin. It labels a variety of mesenchymal cells, including melanocytes, lymphocytes, endothelial cells, and fibroblasts. Non-reactivity of anti-vimentin is often considered more useful than its positive reactivity, since there are a few tumors that do not contain vimentin, e.g. hepatoma and seminoma. Anti-vimentin is also useful as a tissue process control reagent.
    Keine Kreuzreaktivität
    Maus, Ratte (Rattus)
    Aufreinigung
    Purified by Protein A/G
    Immunogen
    Recombinant full-length human vimentin protein
    Klon
    VM452
    Isotyp
    IgG1 kappa
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    Discover our top product VIM Primärantikörper
  • Applikationshinweise

    Positive Control: Raji, Jurkat or HeLa cells. Sarcomas or Melanomas.

    Known Application: Flow Cytometry (1-2 μg/million cells), Western Blot (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (0.5-1.0 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Konzentration
    200 μg/mL
    Buffer
    10 mM PBS with 0.05 % BSA & 0.05 % azide.
    Konservierungsmittel
    Sodium azide
    Vorsichtsmaßnahmen
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Lagerung
    4 °C,-80 °C
    Informationen zur Lagerung
    Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
    Haltbarkeit
    24 months
  • Target
    Vimentin (VIM)
    Andere Bezeichnung
    VIM (VIM Produkte)
    Synonyme
    CTRCT30 antikoerper, cb28 antikoerper, vime antikoerper, vim antikoerper, vim1 antikoerper, vim2 antikoerper, VIM antikoerper, Vimentin antikoerper, vim4 antikoerper, vimentin antikoerper, vimentin L homeolog antikoerper, vimentin S homeolog antikoerper, VIM antikoerper, Vim antikoerper, vim antikoerper, vim.L antikoerper, vim.S antikoerper
    Molekulargewicht
    57-60kDa
    Gen-ID
    7431
    UniProt
    P08670
    Pathways
    Caspase Kaskade in der Apoptose
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