WISP2 Antikörper

Produktnummer ABIN1574004

Produktdetails anti-WISP2 Antikörper

Target: WISP2 (WNT1 Inducible Signaling Pathway Protein 2 (WISP2))

Reaktivität: Bitte anfragen

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser WISP2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA

Aufreinigung: Protein A affinity column

Immunogen: WISP2 full length recombinant protein with GST tag

Klon: 5D11E11

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.

ELISA: 0.05-0.2 µg/mL
Western blot: 0.1-1.0 µg/mL
Other applications: user-optimized

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Buffer: PBS, pH 7.4, containing 0.02 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.

Details zu WISP2

Target: WISP2 (WNT1 Inducible Signaling Pathway Protein 2 (WISP2))

Andere Bezeichnung: WISP2 (WISP2 Produkte)

Synonyme: MGC84196 antikoerper, WISP2 antikoerper, ccn5 antikoerper, CCN5 antikoerper, CT58 antikoerper, CTGF-L antikoerper, Ccn5 antikoerper, Crgr4 antikoerper, Ctgfl antikoerper, Rcop1 antikoerper, WNT1 inducible signaling pathway protein 2 antikoerper, WNT1 inducible signaling pathway protein 2 S homeolog antikoerper, WISP2 antikoerper, wisp2.S antikoerper, wisp2 antikoerper, Wisp2 antikoerper

Hintergrund: WISP-2 is a member of the connective tissue growth factor/cysteine-rich 61/nephroblastoma overexpressed (nov) (CCN) family and is upregulated in the mouse mammary epithelial cell line C57MG transformed by Wnt-1 and in several non-invasive human breast tumor cell lines. WISP-2 expression is enhanced by important modulators of human breast cancer cell proliferation such as estrogen, progesterone and epidermal growth factor (EGF) in MCF-7 cells. These effects, inhibited by appropriate antagonists, indicate that steroids and growth factor-induced upregulation of WISP-2 may be mediated through receptors. WISP2 gene may be downstream in the WNT1 signaling pathway that is relevant to malignant transformation. Its expression in colon tumors is reduced while the other two WISP members are overexpressed in colon tumors. It is expressed at high levels in bone tissue, and may play an important role in modulating bone turnover.

Pathways: WNT Signalweg, Growth Factor Binding