WNT3A Antikörper

Produktnummer ABIN1501756

Produktdetails anti-WNT3A Antikörper

Target: WNT3A (Wingless-Type MMTV Integration Site Family, Member 3A (WNT3A))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser WNT3A Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Marke: IHC-plus™

Spezifität: Human WNT-3a

Aufreinigung: Immunoaffinity purified

Immunogen: E.coli derived recombinant Human WNT-3a.

Type of Immunogen: Cell extract

Anwendungsinformationen

Applikationshinweise: Approved: ELISA (0.5 - 2 μg/mL), IHC, IHC-P (5 μg/mL), WB (0.1 - 0.2 μg/mL)

Usage: Immunohistochemistry: LS-B8210 in an IHC-plus antibody, validated by our pathologists for use in IHC against formalin-fixed, paraffin-embedded human tissues (IHC validation procedure). A concentration of 5 μg/mL is recommended. ELISA: To detect hWNT-3a by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5-2 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human WNT-3a (LS-C104698) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hWNT-3a. Western Blot: To detect hWNT-3a by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hWNT-3a is 1.5-3 ng/lane, under either reducing or non-reducing conditions.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Sterile water

Konzentration: Lot specific

Buffer: Lyophilized from PBS,  pH 7.2

Handhabung: Avoid freeze/thaw cycles.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store Lyophilized at room temperature up to 1 month
Reconstituted for up to 2 weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.

Details zu WNT3A

Target: WNT3A (Wingless-Type MMTV Integration Site Family, Member 3A (WNT3A))

Andere Bezeichnung: WNT3A (WNT3A Produkte)

Synonyme: WNT3A antikoerper, Wnt-3a antikoerper, vt antikoerper, Zwnt[a] antikoerper, wnt3 antikoerper, wnt3 l antikoerper, wnt3l antikoerper, wnt[a] antikoerper, Xwnt-3a antikoerper, wnt-3a antikoerper, wnt3a-A antikoerper, xwnt3a antikoerper, WNT-3A antikoerper, WNT3 antikoerper, wingless-type MMTV integration site family, member 3A antikoerper, Wnt family member 3A antikoerper, wingless-type MMTV integration site family member 3A L homeolog antikoerper, WNT3A antikoerper, Wnt3a antikoerper, wnt3a antikoerper, wnt3a.L antikoerper

Hintergrund: Name/Gene ID: WNT3A
Family: WNT

Synonyms: WNT3A, Protein Wnt-3a

Gen-ID: 89780

UniProt: P56704

Pathways: WNT Signalweg, Regulation of Muscle Cell Differentiation, Regulation of Cell Size, Positive Regulation of Endopeptidase Activity