RPA1 Antikörper (AA 201-300) (Alexa Fluor 350)

Produktnummer ABIN1390059

Produktdetails anti-RPA1 Antikörper (Alexa Fluor 350)

Target: RPA1 (Replication Protein A1, 70kDa (RPA1))

Bindungsspezifität: AA 201-300

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser RPA1 Antikörper ist konjugiert mit Alexa Fluor 350

Applikation: Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p))

Kreuzreaktivität: Human

Homologie: Mouse,Rat,Dog,Pig,Rabbit

Aufreinigung: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human RPA70

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: FCM 1:20-100
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Konservierungsmittel: ProClin

Vorsichtsmaßnahmen: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Haltbarkeit: 12 months

Details zu RPA1

Target: RPA1 (Replication Protein A1, 70kDa (RPA1))

Andere Bezeichnung: RPA70 (RPA1 Produkte)

Synonyme: HSSB antikoerper, MST075 antikoerper, REPA1 antikoerper, RF-A antikoerper, RP-A antikoerper, RPA70 antikoerper, Cb1-727 antikoerper, wu:fi14b08 antikoerper, zgc:55337 antikoerper, zgc:77092 antikoerper, RPA1 antikoerper, hssb antikoerper, mst075 antikoerper, repa1 antikoerper, rf-a antikoerper, rp-a antikoerper, rpa antikoerper, rpa70 antikoerper, LOC100230990 antikoerper, 5031405K23Rik antikoerper, 70kDa antikoerper, AA589576 antikoerper, AW557552 antikoerper, Rpa antikoerper, CG9633 antikoerper, D-RPA70 antikoerper, D-SSB antikoerper, DRP-A antikoerper, DmRPA antikoerper, Dmel\\CG9633 antikoerper, RPA antikoerper, RPA 70 antikoerper, RPA 70 kDa antikoerper, RpA70 antikoerper, Rpa-70 antikoerper, Ssb-70 antikoerper, dRP-A antikoerper, dmrpa1 antikoerper, i164 antikoerper, replication protein A1 antikoerper, replication protein A1, 70kDa antikoerper, replication protein A1 L homeolog antikoerper, Replication Protein A 70 antikoerper, RPA1 antikoerper, Rpa1 antikoerper, rpa1 antikoerper, rpa1.L antikoerper, RpA-70 antikoerper

Hintergrund:

Synonyms: Dmrpa1, Drosophila Replication Protein A, DRPA, HSSB, Human single stranded DNA binding protein, MST075, MSTP075, p70 antibody REPA1, Replication factor A, Replication factor A protein 1, Replication protein A 70 kDa DNA-binding subunit, Replication protein A 70 kDa DNA binding subunit, Replication protein A1 70 kDa, Replication protein A1, RF A, RF-A protein 1, RFA, RFA1_HUMAN, RP A, RP-A p70, RPA 70, RPA, RPA1, Single stranded binding protein 70, Single-stranded DNA-binding protein.

Background: The single-stranded-DNA-binding proteins (SSBs) are essential for DNA function in prokaryotic and eukaryotic cells, mitochondria, phages and viruses. Replication protein A (RPA), a highly conserved eukaryotic protein, is a heterotrimeric SSB. RPA plays an important role in DNA replication, recombination and repair. The binding of human RPA (hRPA) to DNA involves molecular polarity in which initial hRPA binding occurs on the 5' side of an ssDNA substrate and then extends in the 3' direction to create a stably bound hRPA. RPA is a major damage-recognition protein involved in the early stages of nucleotide excision repair. It can also play a role in telomere maintenance. The RPA 70 kDa subunit binds to ssDNA and mediates interactions with many cellular and viral proteins. The DNA binding domain lies in the middle of RPA 70 kDa subunit and comprises two structurally homologous subdomains oriented in tandem. RPA contains a conserved four cysteine-type zinc-finger motif, which mediates the transition of RPA-ssDNA interaction to a stable RPA-ssDNA complex in a redox-dependent manner.

Pathways: Telomere Maintenance, DNA Reparatur, Mitotic G1-G1/S Phases, DNA Replication, Chromatin Binding, Synthesis of DNA