Dendritic Cells Antikörper (FITC)

Produktnummer ABIN133418

Produktdetails

Target: Dendritic Cells

Reaktivität: Ratte

Wirt: Maus

Klonalität: Monoklonal

Konjugat: FITC

Applikation: Flow Cytometry (FACS)

Spezifität: Rat lymphoid and non-lymphoid dendritic cells

Aufreinigung: Purified from ascites via Protein G Chromatography

Klon: OX-62

Isotyp: IgG1

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Konzentration: 0.1 mg/ml

Buffer: FITC conjugated Ig buffered in PBS, 0.02% NaN and EIA grade BSA as a stabilizing protein to bring total protein concentration to 4-5 mg/ml.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Antigendetails

Target: Dendritic Cells

Andere Bezeichnung: Dendritic Cell

Hintergrund: ABIN133165 (OX-62) monoclonal antibody was raised against density gradient-enriched rat veiled (dendritic) cells obtained from lymph. This antibody only labeled cells characteristic of veiled cells the antigen recognized by ABIN133165 is expressed at low to moderate levels by a subset of spleen, thymus and bone marrow dendritic cells. In lymphoid tissues, the labeling correlated with dendritic cells (DC), however in skin, MHC Class II cells were ABIN133165 , while other CD3 cells with dendritic morphology were strongly stained with ABIN133165. Therefore, it seems that this antibody may be restricted to DC and probably T cells. (This has since been contradicted by the fact that the CD3 cells with dendritic morphology were confirmed to stain negative for ABIN133204 (OX-19), and are therefore not T cells.) The ABIN133165 antigen has the biochemical characteristics of an integrin (ABIN133165 recognizes the -like subunit) as determined by SDS-PAGE. This monoclonal antibody is useful for cell purification, delineating lineage relationships among cells with dendritic morphology and for molecular studies. The OX-62 antigen is not essential for T cell activation. The ABIN133165 clone has been use in flow cytometry, immunohistochemistry, immunoprecipitation and cell surface radioiodination.