Dendritic Cells Antikörper

Produktnummer ABIN112003

Produktdetails

Target: Dendritic Cells

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Unkonjugiert

Applikation: Immunohistochemistry (Frozen Sections) (IHC (fro))

Aufreinigung: Protein A chromatography.

Immunogen: Circulating immune complexes of a patient with Hodgkin's disease.

Klon: Ki-M4

Isotyp: IgG2a

Anwendungsinformationen

Applikationshinweise: Immunohistochemistry on frozen sections: use at 0.5 μg/mL (1: 400).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Protokoll: Protocol with frozen, ice-cold acetone-fixed sections: The whole procedure is performed at room temperature1. Wash in PBS2. Block endogenous peroxidase3. Wash in PBS4. Block with 10% normal goat serum in PBS for 30min. in a humid chamber5. Incubate with primary antibody (dilution see datasheet) for 1h in a humid chamber6. Wash in PBS7. Incubate with secondary antibody (peroxidase-conjugated goat anti mouse IgG+IgM(H+L) minimal-cross reaction to human) for 1h in a humid chamber8. Wash in PBS9. Incubate with AEC substrate (3-amino-9-ethylcarbazol) for 12min. 10. Wash in PBS11. Counterstain with Mayer's hemalum

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Rekonstitution: Restore with 0.5 mL distilled water.

Konzentration: 0.2 mg/mL

Buffer: PBS buffer with 0.02 % sodium azide as preservative and 10 mg/mL BSA as stabilizer.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: -20 °C

Antigendetails

Target: Dendritic Cells

Hintergrund: Monoclonal antibody Ki-M4 detects the accessory B-cell macrophages in lymphoid organs.Synonyms: Dendritic cell marker