ECT2 Antikörper (pThr790)

Produktnummer ABIN129688

Produktdetails anti-ECT2 Antikörper

Target: ECT2 (Epithelial Cell Transforming Sequence 2 Oncogene (ECT2))

Bindungsspezifität: AA 785-795, pThr790

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser ECT2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunoprecipitation (IP)

Kreuzreaktivität: Maus, Ratte (Rattus), Zebrafisch (Brachydanio rerio), Schimpanse, Huhn, Hund

Produktmerkmale: Concentration Definition: by UV absorbance at 280 nm

Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids 785-795 of human ECT2 protein.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: This affinity-purified antibody has been tested for use in ELISA, western blot and immunoprecipitation.  Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 101 kDa in size by western blotting in the appropriate cell lysate or extract.  Less than 2% reactivity is observed against the non-phosphorylated form of the immunizing peptide.  This antibody is believed to be phospho specific for ECT2 phosphorylated at the pT790 residue.  Experiments to generate more conclusive evidence are currently under preparation.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1.1 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Details zu ECT2

Target: ECT2 (Epithelial Cell Transforming Sequence 2 Oncogene (ECT2))

Andere Bezeichnung: ECT2 (ECT2 Produkte)

Synonyme: ECT2 antikoerper, arhgef31 antikoerper, xect2 antikoerper, AI528536 antikoerper, mKIAA4037 antikoerper, ARHGEF31 antikoerper, ect2 antikoerper, epithelial cell transforming 2 antikoerper, ect2 oncogene antikoerper, epithelial cell transforming 2 S homeolog antikoerper, ECT2 antikoerper, ect2 antikoerper, Ect2 antikoerper, ect2.S antikoerper

Hintergrund: This antibody is designed, produced, and is suitable for Cancer, Immunology and Nuclear Signaling research. ECT2, also known as epithelial cell transforming sequence 2 oncogene, was originally isolated as a transforming gene from epithelial cells. ECT2 catalyzes guanine nucleotide exchange on the small GTPases, RhoA, Rac1, and Cdc42.  ECT2 may be phos-phorylated during G₂ and M phases, and phosphorylation may be required for its exchange activity. Unlike other known guanine nucleotide exchange factors for Rho GTPases, ECT2 exhibits nuclear localization in interphase, spreads throughout the cytoplasm in prometaphase, and is condensed in the midbody during cytokinesis. Expression of dominant-negative ECT2 or microinjection of affinity-purified anti-ECT2 antibody into interphase cells strongly inhibits cytokinesis. These results suggest that ECT2 is an important link between the cell cycle machinery and Rho signaling pathways involved in the regulation of cell division.  Phosphorylation at T790 or S375 significantly affects the catalytic activity of ECT2.
Synonyms: ECT 2 antibody, ECT2 protein antibody, Epithelial cell transforming sequence 2 antibody

Gen-ID: 1894, 21735572

UniProt: Q9H8V3

Pathways: Neurotrophin Signalübertragung, Cell-Cell Junction Organization