RAF1 Antikörper (pSer621)

Produktnummer ABIN126881

Produktdetails anti-RAF1 Antikörper

Target: RAF1 (V-Raf-1 Murine Leukemia Viral Oncogene Homolog 1 (RAF1))

Bindungsspezifität: pSer621

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser RAF1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Enzyme Immunoassay (EIA)

Spezifität: This antibody specifically recognizes c-raf phosphorylated at serine 621. The antibody is an important tool to analyse the contribution of the serine 621 phosphorylation site to c-raf activity.

Produktmerkmale: Synonyms: C-RAF, Raf-1, cRaf, RAF proto-oncogene serine/threonine-protein kinase

Aufreinigung: Size exclusion chromatography

Immunogen: Synthetic phosphopeptide conjugated to KLH

Klon: 6B4

Isotyp: IgG2a

Anwendungsinformationen

Applikationshinweise: Western Blot: 0.5 μg/mL for HRPO/ECL detection. Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubationbuffer. ELISA: 0.05 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Rekonstitution: Restore with 1 mL H2O (15 min, RT).

Buffer: 1 mL2 x PBS / 0.09 % Na-azide / PEG and Sucrose

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C/-80 °C

Informationen zur Lagerung: Store lyophilized (preferably in a desiccator) at -20 °C and reconstituted (aliquote andfreeze in liquid nitrogen) at -80 °C. Avoid repeated freezing and thawing. Thaw aliquots at 37 °C. Thawed aliquots may be stored at 4 °C up to 3 months.
Shelf life: one year from despatch.

Haltbarkeit: 12 months

Details zu RAF1

Target: RAF1 (V-Raf-1 Murine Leukemia Viral Oncogene Homolog 1 (RAF1))

Andere Bezeichnung: RAF1 (RAF1 Produkte)

Synonyme: CRAF antikoerper, NS5 antikoerper, Raf-1 antikoerper, c-Raf antikoerper, c-raf antikoerper, craf antikoerper, raf1 antikoerper, RAF1 antikoerper, 6430402F14Rik antikoerper, AA990557 antikoerper, BB129353 antikoerper, Craf1 antikoerper, D830050J10Rik antikoerper, v-Raf antikoerper, raf antikoerper, cRaf antikoerper, Raf-1 proto-oncogene, serine/threonine kinase antikoerper, Raf-1 proto-oncogene, serine/threonine kinase a antikoerper, v-raf-leukemia viral oncogene 1 antikoerper, Raf-1 proto-oncogene, serine/threonine kinase S homeolog antikoerper, RAF1 antikoerper, Raf1 antikoerper, raf1a antikoerper, raf1 antikoerper, raf1.S antikoerper

Hintergrund: The c-raf protein is an important kinase in the transmission of extracellular stimuli to the nucleus. Mitogenic stimulaton of cells induces hyperphosphorylation of c-raf in multiple serine, threonine and tyrosine residues. Phosphorylation of serines 43, 259 and 621 has been implicated in the negative regulation of raf. Phosphorylation of serine 43 interferes with ras binding, serine 259 and serine 621 represent binding sites for the 14-3-3 proteins.Synonyms: C-RAF, RAF proto-oncogene serine/threonine-protein kinase, Raf-1, cRaf

Gen-ID: 5894

UniProt: P04049

Pathways: MAPK Signalweg, RTK Signalweg, Fc-epsilon Rezeptor Signalübertragung, Neurotrophin Signalübertragung, cAMP Metabolic Process, Stem Cell Maintenance, Hepatitis C, Autophagie, Signaling of Hepatocyte Growth Factor Receptor, VEGF Signaling, BCR Signaling