JNK2 Antikörper

Produktnummer ABIN126823

Dieses Maus Monoklonal-Antikörper erkennt spezifisch JNK2 in WB, EIA und IHC (fro). Er zeigt eine Reaktivität gegenüber Human, Maus, Ratte und Hund.

Kurzübersicht für JNK2 Antikörper (ABIN126823)

Target: JNK2 (MAPK9) (Mitogen-Activated Protein Kinase 9 (MAPK9))

Reaktivität: Human, Maus, Ratte, Hund

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser JNK2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Sections) (IHC (fro))

Klon: 12C5

Produktdetails anti-JNK2 Antikörper

Spezifität: This antibody specifically interacts with the N-terminus of SAPK1α/jnk2 kinases.

Produktmerkmale: Synonyms: JNK-55, PRKM9, MAP kinase 9, Mitogen-activated protein kinase 9, Stress-activated proteinkinase JNK2, c-Jun N-terminal kinase 2

Aufreinigung: Subsequent thiophilic adsorbtion and Size Exclusion Chromatography

Bestandteile: incl. positive Control

Immunogen: Synthetic peptide conjugated to KLH.

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Western Blot: 0.5 μg/mL for HRPO/ECL detection. Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubationbuffer. ELISA: 0.05 μg/mL. Immunohistochemistry on frozen sections. Positive Control: Cell lysate from untreated A431 cells. (See Protocol)
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Protokoll: Positive Control Cell Lysate (A431 Untreated)

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Rekonstitution: Restore with 1 mL H2O (15 min, RT).

Buffer: 1 mLPBS containing 0.09 % Sosium Azide, PEG and Sucrose

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C/-80 °C

Informationen zur Lagerung: Store lyophilized (preferably in a desiccator) at -20 °C and reconstituted (aliquote andfreeze in liquid nitrogen) at -80 °C. Avoid repeated freezing and thawing. Thaw aliquots at 37 °C. Thawed aliquots may be stored at 4 °C up to 3 months.
Shelf life: One year from despatch.

Haltbarkeit: 12 months

Details zu JNK2

Target: JNK2 (MAPK9) (Mitogen-Activated Protein Kinase 9 (MAPK9))

Andere Bezeichnung: MAPK9 / JNK2

Hintergrund: Stress-activated Protein Kinases (SAPKs) are strongly activated in response to adverse stimuli such as heat and osmotic shock, UV light and other DNA-damaging reagents, and inhibitors of protein synthesis. They are also activated strongly in response to agonists that are released or produced under conditions of stress, such as proinflammatory cytokines. The SAPK1/jnk family consists of 3 isoforms: SAPK1α/jnk2, SAPK1β/jnk3, SAPK1γ/jnk1.Synonyms: JNK-55, MAP kinase 9, Mitogen-activated protein kinase 9, PRKM9, Stress-activated protein kinase JNK2, c-Jun N-terminal kinase 2

Molekulargewicht: 54 kDa

Gen-ID: 5601

UniProt: P45984

Pathways: MAPK Signalweg, WNT Signalweg, TLR Signalweg, Fc-epsilon Rezeptor Signalübertragung, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Endopeptidase Activity, Hepatitis C, Toll-Like Receptors Cascades, BCR Signaling, S100 Proteine