FYN Antikörper (AA 7-176)

Produktnummer ABIN120706

Produktdetails anti-FYN Antikörper

Target: FYN (FYN Oncogene Related To SRC, FGR, YES (FYN))

Bindungsspezifität: AA 7-176

Reaktivität: Human, Maus

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser FYN Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunofluorescence (IF)

Spezifität: The antibody reacts with p59fyn (Fyn), a non receptor protein tyrosine kinase (~59 kDa), which belongs to the Src family of cytoplasmic tyrosine kinases.

Aufreinigung: Protein A Affinity Chromatography

Immunogen: Bacterially expressed recombinant fragment of human Fyn (aa 7-176)

Klon: FYN-01

Isotyp: IgG2b

Anwendungsinformationen

Applikationshinweise: Immunoprecipitation (Positive control: J77 T cell line, Preparation of cell lysate: 30 min on ice (orbital incubator) in lysing buffer, lysing buffer with N-dodecyl beta-D-maltoside (20 mM Tris/Cl, 100 mM NaCl pH 8,2, 1 % laurylmaltosid (w/v), 50 mM NaF). Application note: The antibody FYN-01 apparently gives very specific signal (by 59 kDa), and nicely immunoprecipitates Fyn from cell lysates.) Western Blotting (2 mg/mL, Positive control: JURKAT human T cell leukemia cell line). Immunocytochemistry. Immunohistochemistry (paraffin sections): 5 m g/mL. Positive tissue: brain
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: Phosphate buffered saline (PBS) with 15 mM Sodium Azide, approx. pH 7.4

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf Life: one year from despatch. General.

Haltbarkeit: 12 months

Details zu FYN

Target: FYN (FYN Oncogene Related To SRC, FGR, YES (FYN))

Andere Bezeichnung: FYN (FYN Produkte)

Synonyme: im:6908851 antikoerper, si:dkey-119m7.5 antikoerper, zgc:109996 antikoerper, c-fyn antikoerper, cfyn-a antikoerper, SLK antikoerper, SYN antikoerper, p59-FYN antikoerper, PKC antikoerper, fyn antikoerper, zgc:86720 antikoerper, AI448320 antikoerper, AW552119 antikoerper, FYN proto-oncogene, Src family tyrosine kinase antikoerper, FYN proto-oncogene, Src family tyrosine kinase S homeolog antikoerper, FYN proto-oncogene, Src family tyrosine kinase b antikoerper, FYN proto-oncogene, Src family tyrosine kinase L homeolog antikoerper, FYN proto-oncogene, Src family tyrosine kinase a antikoerper, Fyn proto-oncogene antikoerper, FYN antikoerper, fyn.S antikoerper, fynb antikoerper, fyn.L antikoerper, Fyn antikoerper, fyna antikoerper

Hintergrund: Fyn is a membrane-associated, non-receptor protein tyrosine kinase approximately 59 kDa, which belongs to the Src family of cytoplasmic tyrosine kinases. Fyn is very strongly similar to mouse Fyn, v-yes and c-src. Fyn is expressed predominately in tissues of neuronal and hematopoietic origin. Neuronal Fyn and hematopoietic Fyn differ at the junction of the SH2 and kinase domains due to tissue specific alternative splicing. Fyn has been shown to be involved in B cell and T cell activation as well as keratinocyte differentiation. In T cells, Fyn associates with the T-cell antigen receptor and Thy1.Synonyms: Proto-oncogene tyrosine-protein kinase Fyn, Protooncogene Syn, SLK, p59-Fyn

Gen-ID: 2534

UniProt: P06241

Pathways: JAK-STAT Signalweg, T-Zell Rezeptor Signalweg, Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Neurotrophin Signalübertragung, Feeding Behaviour, CXCR4-mediated Signaling Events, Signaling Events mediated by VEGFR1 and VEGFR2, Activated T Cell Proliferation, Thromboxane A2 Receptor Signaling