Interferon gamma Antikörper

Produktnummer ABIN1177279

Produktdetails anti-Interferon gamma Antikörper

Target: Interferon gamma (IFNG)

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Interferon gamma Antikörper ist unkonjugiert

Applikation: ELISA, Neutralization (Neut)

Marke: BD Pharmingen™

Produktmerkmale: Neutralization Activity: This antibody has been reported to be useful for the neutralization of human IFN-gamma anti-viral activity. Neutralization activity may be measured with a encphalomyocarditis viral (EMCV) resistance assay using 1 ng/mL recombinant human IFN-gamma (Cat. No. 554617) with A549 indicator cells.
50% Neutralization (ND50) at 0.25 - 2.0 µg/mL
> 90% Neutralization at 5 - 50 myg/mL

Aufreinigung: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Sterilität: 0.2 μm filtered

Endotoxin-Niveau: Endotoxin level is ≤ 0.01 EU/μg (≤ 0.001 ng/μg) of protein as determined by the LAL assay.

Immunogen: Recombinant human IFN-gamma

Klon: NIB42

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise: ELISA: Purified NIB42 antibody can be useful as a capture antibody in sandwich ELISA for measuring human IFN-gamma protein levels. Purified NIB42 antibody can be paired with biotinylated 4S.B3 (Cat. No. 554550) as the detection antibody along with recombinant human IFN-gamma (Cat. No. 554617) as the standard. Investigators are highly encouraged, when measuring human IFN-gamma in serum or plasma, to utilize BD OptEIA™ Human IFN-gamma ELISA Set (Cat. No. 555142) or BD OptEIA™ Human IFN-gamma ELISA Kit II (Cat. No. 550612).

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1.0 mg/mL

Buffer: No azide/low endotoxin: Aqueous buffered solution containing no preservative, 0.2μm sterile filtered.

Konservierungsmittel: Azide free

Lagerung: 4 °C

Informationen zur Lagerung: Store undiluted at 4°C. This preparation contains no preservatives, thus it should be handled under aseptic conditions.

Referenzen für anti-Interferon gamma Antikörper (ABIN1177279)

Green, Yeh, Overall: "Rapid, quantitative, semiautomated assay for virus-induced and immune human interferons." in: Journal of clinical microbiology, Vol. 12, Issue 3, pp. 433-8, (1981) (PubMed).

Details zu Interferon gamma

Target: Interferon gamma (IFNG)

Andere Bezeichnung: IFN-gamma (IFNG Produkte)

Synonyme: IFG antikoerper, IFI antikoerper, IFN-g antikoerper, Ifg antikoerper, IFNG2 antikoerper, IFN-gamma antikoerper, IFN-G antikoerper, IFNG antikoerper, IFNgamma antikoerper, TCRalpha antikoerper, INF-G antikoerper, ifng antikoerper, interferon gamma antikoerper, interferon, gamma 1-2 antikoerper, IFNG antikoerper, Ifng antikoerper, ifng1-2 antikoerper

Hintergrund: The NIB42 antibody reacts with human interferon-gamma (IFN-gamma). The immunogen used to generate the NIB42 hybridoma was recombinant human IFN-gamma. This is a neutralizing antibody.

Pathways: Interferon-gamma Pathway, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, ER-Nucleus Signaling, Regulation of Carbohydrate Metabolic Process, Protein targeting to Nucleus, Autophagie