MMP 9 Antikörper (AA 213-399) (Biotin)

Produktnummer ABIN1173110

Produktdetails anti-MMP 9 Antikörper (Biotin)

Target: MMP 9 (MMP9) (Matrix Metallopeptidase 9 (Gelatinase B, 92kDa Gelatinase, 92kDa Type IV Collagenase) (MMP9))

Bindungsspezifität: AA 213-399

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser MMP 9 Antikörper ist konjugiert mit Biotin

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Sequenz: MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- GKGVVVPT RFGNADGAAC HFPFIFEGRSYSACTTDGRS DGLPWCSTTA NYDTDDRFGF CPSERLYTQD GNADGKPCQF PFIFQGQSYS ACTTDGRSDG YRWCATTANY DRDKLFGFCP TRADSTVMGG NSAGELCVFP FTFLGKEYST CTSEGRGDGR LWCATTSNFD SDKKWGFCPD QGYSLFLVA

Spezifität: It has been selected for its ability to recognize MMP9 in immunohistochemical staining and Western blotting.

Aufreinigung: Affinity Chromatography

Immunogen: Recombinant MMP9 expressed in E.coli.
The antibody is a rabbit polyclonal antibody raised against MMP9 conjugated to biotin.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Optimal working dilutions must be determined by end user.

Kommentare:

Content: The quality control contains recombinant MMP9 (Gly213~Ala399) disposed in loading buffer.
Usage: 10 µL per well when 3,3'-Diaminobenzidine(DAB) as the substrate. 5 µL per well when used in enhanced chemilumescent (ECL).
Note: The quality control is specifically manufactured as the positive control.Not used for other purposes.
Loading Buffer: 100 mM Tris(pH8.8), 2 % SDS, 200 mM NaCl, 50 % glycerol,BPB 0.01 % , NaN3 0.02 % .

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: Supplied as solution form in PBS, pH7.4, containing 0.02 % NaN3, 50 % glycerol.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Handhabung: Avoid repeated freeze/thaw cycles

Lagerung: 4 °C

Informationen zur Lagerung: Store at 2-8 °C for one month. Aliquot and store at -80 °C for 12 months.

Haltbarkeit: 12 months

Details zu MMP 9

Target: MMP 9 (MMP9) (Matrix Metallopeptidase 9 (Gelatinase B, 92kDa Gelatinase, 92kDa Type IV Collagenase) (MMP9))

Andere Bezeichnung: Matrix Metalloproteinase 9 (MMP9) (MMP9 Produkte)

Synonyme: CLG4B antikoerper, GELB antikoerper, MANDP2 antikoerper, MMP-9 antikoerper, AW743869 antikoerper, B/MMP9 antikoerper, Clg4b antikoerper, pro-MMP-9 antikoerper, mmp-9 antikoerper, fgMMP-9 antikoerper, mmp9 antikoerper, ZFMMP-9 antikoerper, wu:fb02g06 antikoerper, wu:fb07b05 antikoerper, wu:fi98c09 antikoerper, wu:fj05a08 antikoerper, zgc:64165 antikoerper, clg4b antikoerper, gelb antikoerper, mandp2 antikoerper, matrix metallopeptidase 9 antikoerper, collagenase antikoerper, matrix metalloproteinase 9 antikoerper, matrix metallopeptidase 9 S homeolog antikoerper, MMP9 antikoerper, RB3913 antikoerper, BPSS0666 antikoerper, Sbal_3732 antikoerper, Bcer98_0486 antikoerper, Shew185_0630 antikoerper, Shal_3056 antikoerper, Sbal195_0657 antikoerper, Lbys_3550 antikoerper, Palpr_2084 antikoerper, Mmp9 antikoerper, mmp9 antikoerper, mmp9.S antikoerper

Pathways: Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Immune Effector Process, CXCR4-mediated Signaling Events