Ovalbumin Antikörper

Produktnummer ABIN116785

Produktdetails anti-Ovalbumin Antikörper

Target: Ovalbumin (OVA)

Reaktivität: Huhn

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Ovalbumin Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunoprecipitation (IP), Enzyme Immunoassay (EIA)

Spezifität: Assay by Immunoelectrophoresis resulted in a single precipitin arc against purified and partially purified Ovalbumin [Hen Egg White]. Cross reactivity against Ovalbumin from other tissues and species may occur but have not been specifically determined.

Aufreinigung: Delipidation and Defibrination

Immunogen: Ovalbumin from Hen Egg White.

Anwendungsinformationen

Applikationshinweise: Suitable for Immunoblotting (Western: 1/500-1/5000 or Dot blot), ELISA (1/5000-1/20000),Immunoprecipitation (1/100) and most Immunological methods requiring high titer andspecificity. Recommended Dilutions: This product has been assayed against 1.0 μg of Ovalbumin [Hen Egg White] in a standardsandwich ELISA using Peroxidase conjugated Affinity Purified anti-Rabbit IgG [H&L] (Goat)(Cat#R1364HRP) and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as asubstrate for 30 minutes at room temperature. A working dilution of 1/90,000 to 1/450,000of the reconstitution concentration is suggested for this product.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Rekonstitution: Restore with 2.0 mL of deionized water (or equivalent).

Konzentration: 80.0 mg/mL (by Refractometry)

Buffer: 0.02 M Potassium Phosphate, 0.12 M Sodium Chloride, pH 7.2, 0.01 % (w/v) Sodium Azide, None

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Prior to reconstitution store at 2-8 °C. Following reconstitution store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Details zu Ovalbumin

Target: Ovalbumin (OVA)

Andere Bezeichnung: Ovalbumin (OVA Produkte)

Synonyme: 1600019A21Rik antikoerper, R86 antikoerper, SPI-CI antikoerper, Spi10 antikoerper, ovalbumin antikoerper, NK13 antikoerper, Spi12 antikoerper, BC052216 antikoerper, NK21 antikoerper, NK21L1 antikoerper, Spi13 antikoerper, NK26 antikoerper, Spi14 antikoerper, AW540195 antikoerper, AT2 antikoerper, Spi9 antikoerper, EID antikoerper, SPI3B antikoerper, Gm11396 antikoerper, OTTMUSG00000000720 antikoerper, SERPINB14 antikoerper, C76171 antikoerper, C76174 antikoerper, SPI3C antikoerper, SPIC antikoerper, serine (or cysteine) peptidase inhibitor, clade B, member 9b antikoerper, serine (or cysteine) peptidase inhibitor, clade B, member 6b antikoerper, serine (or cysteine) peptidase inhibitor, clade B, member 9f antikoerper, serine (or cysteine) peptidase inhibitor, clade B, member 9e antikoerper, serine (or cysteine) peptidase inhibitor, clade B, member 9d antikoerper, serine (or cysteine) peptidase inhibitor, clade B, member 1, pseudogene antikoerper, serine (or cysteine) peptidase inhibitor, clade B, member 6e antikoerper, ovalbumin (SERPINB14) antikoerper, serine (or cysteine) peptidase inhibitor, clade B, member 6c antikoerper, Serpinb9b antikoerper, Serpinb6b antikoerper, Serpinb9f antikoerper, Serpinb9e antikoerper, Serpinb9d antikoerper, Serpinb1-ps1 antikoerper, Serpinb6e antikoerper, OVAL antikoerper, Serpinb6c antikoerper

Hintergrund: Chicken Ovalbumin is the major protein in the "white" of the egg (and a favorite antigen in immunological research). Egg white contains a variety of proteins including ovalbumin, conalbumin, ovomucoid and lysozyme. It belongs to the serpin family and the Ov serpin subfamily. Ovalbumin can cause an allergic reaction in humans. Ovalbumin has been implicated in the development of the egg shell. Immunohistochemistry revealed that ovalbumin is found only in the mammillary bodies of decalcified shell, and is not distributed throughout the shell matrix. These results indicate that ovalbumin is present during the initial phase of shell formation and becomes incorporated into the protein matrix of the mammillary bodies. However, it is not yet clear whether ovalbumin at this site plays a specific role in shell mineralisation. Anti Ovalbumin Antibody recognizes ovalbumin that is the main protein found in egg white. Ovalbumin makes up 60-65 % of the total protein. Ovalbumin displays sequence and three-dimensional homology to the serpin superfamily, but unlike most serpins it is not a serine protease inhibitor. The function of ovalbumin is unknown, although it is presumed to be a storage protein.Synonyms: Allergen Gal d II, Egg albumin, Plakalbumin, SERPINB14

Gen-ID: 396058

NCBI Accession: NP_990483

UniProt: P01012