Ovalbumin Antikörper (Biotin)

Produktnummer ABIN1607759

Produktdetails anti-Ovalbumin Antikörper (Biotin)

Target: Ovalbumin (OVA)

Reaktivität: Huhn

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Ovalbumin Antikörper ist konjugiert mit Biotin

Applikation: Western Blotting (WB), ELISA, Immunoprecipitation (IP)

Kreuzreaktivität (Details): Cross reactivities against Ovalbumin from other sources may occur but have not been specifically determined.

Reinheit: Anti-Ovalbumin Antibody Biotin Conjugated is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Rabbit Serum as well as purified and partially purified Ovalbumin [Hen Egg White].

Endotoxin-Niveau: Low Endotoxin : No

Immunogen: Ovalbumin [Hen Egg White]
Immunogen Type: Native Protein

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Anti-Ovalbumin Antibody Biotin Conjugated is suitable for both ELISA, and for western blotting. In western blots anti-ovalbumin detects a single band of the expected apparent molecular weight. Researchers should determine optimal titers for applications that are not stated below.
ELISA Dilution: 1:30.000
Fluorochrome Protein Value: 10-20
IF Immunoprecipitation Dilution: 1:100
Western Blot Dilution: 1:500 - 1:5.000

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Handhabung: Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Store vial at -20 °C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below.

Haltbarkeit: Expiration date is one (1) year from date of opening.

Details zu Ovalbumin

Target: Ovalbumin (OVA)

Andere Bezeichnung: Ovalbumin (OVA Produkte)

Synonyme: 1600019A21Rik antikoerper, R86 antikoerper, SPI-CI antikoerper, Spi10 antikoerper, ovalbumin antikoerper, NK13 antikoerper, Spi12 antikoerper, BC052216 antikoerper, NK21 antikoerper, NK21L1 antikoerper, Spi13 antikoerper, NK26 antikoerper, Spi14 antikoerper, AW540195 antikoerper, AT2 antikoerper, Spi9 antikoerper, EID antikoerper, SPI3B antikoerper, Gm11396 antikoerper, OTTMUSG00000000720 antikoerper, SERPINB14 antikoerper, C76171 antikoerper, C76174 antikoerper, SPI3C antikoerper, SPIC antikoerper, serine (or cysteine) peptidase inhibitor, clade B, member 9b antikoerper, serine (or cysteine) peptidase inhibitor, clade B, member 6b antikoerper, serine (or cysteine) peptidase inhibitor, clade B, member 9f antikoerper, serine (or cysteine) peptidase inhibitor, clade B, member 9e antikoerper, serine (or cysteine) peptidase inhibitor, clade B, member 9d antikoerper, serine (or cysteine) peptidase inhibitor, clade B, member 1, pseudogene antikoerper, serine (or cysteine) peptidase inhibitor, clade B, member 6e antikoerper, ovalbumin (SERPINB14) antikoerper, serine (or cysteine) peptidase inhibitor, clade B, member 6c antikoerper, Serpinb9b antikoerper, Serpinb6b antikoerper, Serpinb9f antikoerper, Serpinb9e antikoerper, Serpinb9d antikoerper, Serpinb1-ps1 antikoerper, Serpinb6e antikoerper, OVAL antikoerper, Serpinb6c antikoerper

Hintergrund: Ovalbumin Antibody Biotin Conjugated is specific for ovalbumin. Ovalbumin (abbreviated OVA) is the main protein found in egg white, making up 60-65 % of the total protein. Ovalbumin displays sequence and three-dimensional homology to the serpin superfamily. The function of ovalbumin is unknown, although it is presumed to be a storage protein or carrier protein. Synonyms: Ovalbumin, Egg albumin. Plakalbumin, Allergen Gal d II, Allergen=Gal d 2

Gen-ID: 396058

UniProt: P01012