NFYB Antikörper (N-Term)

Produktnummer ABIN116537

Produktdetails anti-NFYB Antikörper

Target: NFYB (Nuclear Transcription Factor Y, beta (NFYB))

Bindungsspezifität: N-Term

Reaktivität: Human, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser NFYB Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunoprecipitation (IP), Electrophoretic Mobility-Shift Assay (EMSA), Enzyme Immunoassay (EIA)

Aufreinigung: This product was prepared from monospecific antiserum by delipidation and defibrination.

Immunogen: NF-Y (B subunit specific) peptide corresponding to a region near the N-terminus of the human protein conjugated to Keyhole Limpet Hemocyanin (KLH).

Anwendungsinformationen

Applikationshinweise: Suitable for Immunoprecipitation, Immunoblotting, ELISA and supershift assays. Recommended Dilutions: This product was assayed by immunoblot and found to bereactive against NF-Y (B subunit specific) at a dilution of 1: 2,000 followed by reaction withPeroxidase conjugated Affinity Purified anti-Rabbit IgG [H&L] (Goat). Anti-NF-Y (B subunit specific) is suitable for the detection by Immunoblot of human andmouse NF-Y. Minimal reactivity was observed against the A subunit of NF-Y. This product was also tested in a gel supershift assay and found to be reactive against NF-Yusing 0.5 to 1.0 mLper assay.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 90.0 mg/mL (by Refractometry)

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store original vial at 2-8 °C. After reconstitution store at 2-8 °C for up to one month or (in aliquots) at -20 °C for longer.

Details zu NFYB

Target: NFYB (Nuclear Transcription Factor Y, beta (NFYB))

Andere Bezeichnung: NFYB (NFYB Produkte)

Synonyme: CBF-A antikoerper, CBF-B antikoerper, HAP3 antikoerper, NF-YB antikoerper, NFYB antikoerper, nfyb antikoerper, zgc:110533 antikoerper, zgc:110552 antikoerper, hap3 antikoerper, cbf-a antikoerper, cbf-b antikoerper, CBFB antikoerper, Nfya antikoerper, AA985999 antikoerper, Cbf-A antikoerper, nuclear transcription factor Y subunit beta antikoerper, nuclear transcription factor Y subunit beta L homeolog antikoerper, nuclear transcription factor Y, beta b antikoerper, nuclear transcription factor Y, beta a antikoerper, nuclear transcription factor-Y beta antikoerper, NFYB antikoerper, nfyb.L antikoerper, nfybb antikoerper, nfyba antikoerper, nfyb antikoerper, Nfyb antikoerper

Hintergrund: The Y box is a CCAAT box which is bound by the heteromeric DNA binding protein, NFY (also known as CBF and CP1). Unlike the transcription factors C/EBP and CTF/NF1 which also bind CCAAT-like sequences, NFY exhibits a strict binding requirement for this pentanucleotide sequence. Binding sites for this factor have been described for nearly 30 % of all eukaryotic genes. Y/CCAAT sequences were frequently observed in the promoter-proximal sequences. NF-Y is composed of 3 separate subunits (A,B and C) each of which is required for DNA binding. Each subunit has remained highly conserved throughout evolution. In fact, homologous yeast subunits can substitute for mammalian NF-Y in DNA-binding assays. The conserved core sequences of NF-YB and NF-YC contain a 70 aa region similar to the histone fold motif of nucleosomes H2A and H2B. The unique structure and evolutionary conservation of this transcription factor suggests that it plays a fundamental role in the readout of eukaryotic genetic information.Synonyms: HAP3, Nuclear transcription factor Y subunit beta

Gen-ID: 4801

NCBI Accession: NP_006157

UniProt: P25208

Pathways: Regulation of Lipid Metabolism by PPARalpha