NFYB Antikörper (N-Term)

Produktnummer ABIN104771

Produktdetails anti-NFYB Antikörper

Target: NFYB (Nuclear Transcription Factor Y, beta (NFYB))

Bindungsspezifität: N-Term

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser NFYB Antikörper ist unkonjugiert

Applikation: ELISA

Produktmerkmale: Concentration Definition: by UV absorbance at 280 nm

Immunogen: NF-Y (B subunit) peptide corresponding to a region near the of the human protein conjugated to Keyhole Limpet Hemocyanin (KLH).

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: This product was assayed by immunoblot and found to be reactive against the 25 kDa B subunit of NF-Y at a dilution of 1:500 followed by reaction with Peroxidase conjugated Affinity Purified anti-Rabbit IgG [H&L] Goat) Anti-NF-Y (B subunit specific) is suitable for the detection by immunoblot of human and mouse NF-Y (B subunit specific).  Minimal reaction was observed by immunoblot against the 35 kDa A subunit of NF-Y.  This product was also tested in a gel supershift assay and found to be reactive against human and mouse NF-Y using 2.0 to 4.0 µl per assay.  This product was assayed against NF-Y B subunit peptide in an antibody capture ELISA using Peroxidase conjugated Affinity Purified anti-Rabbit IgG [H&L] (Goat) and ABTS substrate.  A dilution of 1:4000 is suggested from this experiment.  Minimal reactivity was observed by ELISA against the A subunit of NF-Y. 

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1.0 mg/mL

Buffer: 0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Referenzen für anti-NFYB Antikörper (ABIN104771)

Manni, Caretti, Artuso, Gurtner, Emiliozzi, Sacchi, Mantovani, Piaggio: "Posttranslational regulation of NF-YA modulates NF-Y transcriptional activity." in: Molecular biology of the cell, Vol. 19, Issue 12, pp. 5203-13, (2008) (PubMed).

Details zu NFYB

Target: NFYB (Nuclear Transcription Factor Y, beta (NFYB))

Abstract: NFYB Produkte

Synonyme: CBF-A antikoerper, CBF-B antikoerper, HAP3 antikoerper, NF-YB antikoerper, NFYB antikoerper, nfyb antikoerper, zgc:110533 antikoerper, zgc:110552 antikoerper, hap3 antikoerper, cbf-a antikoerper, cbf-b antikoerper, CBFB antikoerper, Nfya antikoerper, AA985999 antikoerper, Cbf-A antikoerper, nuclear transcription factor Y subunit beta antikoerper, nuclear transcription factor Y subunit beta L homeolog antikoerper, nuclear transcription factor Y, beta b antikoerper, nuclear transcription factor Y, beta a antikoerper, nuclear transcription factor-Y beta antikoerper, NFYB antikoerper, nfyb.L antikoerper, nfybb antikoerper, nfyba antikoerper, nfyb antikoerper, Nfyb antikoerper

Hintergrund: Synonyms: Nuclear transcription factor Y subunit B

Gen-ID: 4801

UniProt: P25208

Pathways: Regulation of Lipid Metabolism by PPARalpha