Transferrin Antikörper (N-Term)

Produktnummer ABIN112496

Produktdetails anti-Transferrin Antikörper

Target: Transferrin (TF)

Bindungsspezifität: N-Term

Reaktivität: Human, Ratte, Schwein, Kaninchen

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Transferrin Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Enzyme Immunoassay (EIA), Functional Studies (Func)

Spezifität: This antibody is specific for the N-terminal domain of Transferrin and is known to block the binding of this antibody to Transferrin. HTF-14 recognises a band of approximately 77 kDa under reducing conditions in Human plasma. Clone HTF-14 is not able to recognize receptor bound Transferrin. Clone HTF-14 recognizes a conformational epitope in a loop of the N lobe of Transferrin involving the critical Lys-144 residue, also essential for binding of the N lobe to the transferrin receptor (Mason et al.2009) Transferrin is a 77 kDa plasma protein synthesized by the liver involved with the transport of iron. Each transferrin molecule has two domains, both with facility to carry 2 ferric ions. The iron/transferrin complex is essential for haemoglobin synthesis and certain types of cell division. Negative Species. Bovine, Sheep, Canine (Dog) and Equine (Horse).

Kreuzreaktivität (Details): Species reactivity (expected):Porcine, Rat and Rabbit.
Species reactivity (tested):Human.

Aufreinigung: Affinity Chromatography on Protein A

Immunogen: Purified Porcine Transferrin.

Klon: HTF-14

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: ELISA. Immunofluorescence. Functional Application.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: PBS, 0.09 % Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Details zu Transferrin

Target: Transferrin (TF)

Abstract: TF Produkte

Synonyme: ltf antikoerper, pro1557 antikoerper, pro2086 antikoerper, mgc107777 antikoerper, LOC692564 antikoerper, TF antikoerper, LOC100144362 antikoerper, tf antikoerper, LTF antikoerper, TFEW antikoerper, conalbumin antikoerper, tf-b antikoerper, MGC64306 antikoerper, PRO1557 antikoerper, PRO2086 antikoerper, TFQTL1 antikoerper, AI266983 antikoerper, Cd176 antikoerper, HP antikoerper, Tf antikoerper, Tfn antikoerper, hpx antikoerper, Trf antikoerper, cb285 antikoerper, gavi antikoerper, id:ibd3238 antikoerper, id:ibd3525 antikoerper, sb:cb285 antikoerper, wu:fb57g06 antikoerper, wu:fb62h02 antikoerper, wu:fb63h10 antikoerper, wu:fb64h10 antikoerper, zgc:112154 antikoerper, 143958_at antikoerper, CG6186 antikoerper, Dmel\\CG6186 antikoerper, TSF1 antikoerper, anon-EST:Posey265 antikoerper, tsf1 antikoerper, Pro-TRH antikoerper, IL-5 antikoerper, STF I antikoerper, TRF1 antikoerper, sTF1 antikoerper, sTf antikoerper, tf1 antikoerper, transferrin antikoerper, serotransferrin antikoerper, transferrin (ovotransferrin) antikoerper, transferrin L homeolog antikoerper, melanotransferrin antikoerper, transferrin-a antikoerper, Transferrin 1 antikoerper, thyrotropin releasing hormone antikoerper, interleukin 5 antikoerper, TF antikoerper, LOC477072 antikoerper, tf antikoerper, Tf antikoerper, LOC100144362 antikoerper, tf.L antikoerper, LOC5575625 antikoerper, Trf antikoerper, tfa antikoerper, Tsf1 antikoerper, TRH antikoerper, IL5 antikoerper, LOC101085148 antikoerper, LOC100726872 antikoerper, trf antikoerper

Hintergrund: Transferrin is a monomeric glycoprotein of approximately 77 kDa, which serves as an iron-transporter. In normal plasma, transferrin has a concentration of 25-50 mmol / liter, and is usually about one-third saturated with iron, thus providing a large buffering capacity in case of an acute increase in plasma iron levels. Cells take up transferrin-iron complexes (holotransferrin) using transferrin receptor dimers. Upon binding of holotransferrin, the receptor is internalized by clathrin-mediated endocytosis. Acidification of endosomes by vesicular membrane proton pumps leads to dissociation of iron ions, whereas transferrin (apotransferrin) remains associated with its receptor (CD71) and recycles to the cell surface, where apotransferrin is released upon exposure to normal pH . Internalization of labeled transferrin thus represents an usefull approach to study endocytosis. Serum concentration rises in iron deficiency and pregnancy and falls in iron overload, infection and inflammatory conditions. Iron/transferrin complex is essential in haemoglobin synthesis and for certain types of cell division.Synonyms: Beta-1 metal-binding globulin, Serotransferrin, Siderophilin

Gen-ID: 7018

NCBI Accession: NP_001054

UniProt: P02787

Pathways: Transition Metal Ion Homeostasis