BrdU Antikörper

Produktnummer ABIN112396

Produktdetails anti-BrdU Antikörper

Target: BrdU (Bromodeoxyuridine (BrdU))

Reaktivität: Bitte anfragen

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser BrdU Antikörper ist unkonjugiert

Applikation: Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Aufreinigung: Purified

Immunogen: IIB5 is a mouse monoclonal IgG1 antibody derived by fusion of SP2/0-Ag14 mouse myeloma cells with spleen cells from a BALB/c mouse intraperitoneally immunized with BrdU conjugated to BSA

Klon: IIB5

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: This antibody is suitable for Flow cytometry and Immunohistochemistry on frozen andparaffin-embedded tissues. Recommended dilutions: 1/100-1/200 for Flow cytometry, and for Immunohistochemistrywith avidin-biotinylated horseradish peroxidase complex (ABC) as detection reagent.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: PBS, 0.09 % Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the lyophilized antibody at 2-8 °C for one month or (in aliquots) at -20 °C for longer. When reconstituted the antibody is stable for one month at 2-8 °C.

Details zu BrdU

Target: BrdU (Bromodeoxyuridine (BrdU))

Abstract: BrdU Produkte

Substanzklasse: Chemical

Hintergrund: The immunocytochemical detection of bromodeoxyuridine (BrdU) incorporated into DNA is a powerful tool to study the cytokinetics of normal and neoplastic cells. In vitro or in vivo labeling of tumor cells with the thymidine analogue BrdU and the subsequent detection of incorporated BrdU with specific anti-BrdU monoclonal antibodies is an accurate and comprehensive method to quantitate the degree of DNA-synthesis. BrdU is incorporated into the newly synthezised DNA of the S-phase cells and can thus provide an estimate for the fraction of cells in S-phase. Also dynamic proliferative information (such as the S-phase transit rate and the potential doubling time) can be obtained, by means of bivariate BrdU/DNA flow cytometric analysis.