Transferrin Receptor Antikörper

Produktnummer ABIN111936

Produktdetails anti-Transferrin Receptor Antikörper

Target: Transferrin Receptor (TFRC) (Transferrin Receptor (p90, CD71) (TFRC))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Transferrin Receptor Antikörper ist unkonjugiert

Applikation: Immunohistochemistry (Frozen Sections) (IHC (fro))

Kreuzreaktivität (Details): Species reactivity (tested):Human.

Aufreinigung: Affinity Chromatography.

Immunogen: KG-1 cells

Klon: VIP-1

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Immunohistochemistry on Frozen Sections: 2-4 μg/mL (1/50-1/100). Positive Control: Human tonsil.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Protokoll: Protocol with frozen, ice-cold acetone-fixed sections: The whole procedure is performed at room temperature1. Wash in PBS2. Block endogenous peroxidase3. Wash in PBS4. Block with 10% normal goat serum in PBS for 30min. in a humid chamber5. Incubate with primary antibody (dilution see datasheet) for 1h in a humid chamber6. Wash in PBS7. Incubate with secondary antibody (peroxidase-conjugated goat anti mouse IgG+IgM(H+L) minimal-cross reaction to human) for 1h in a humid chamber8. Wash in PBS9. Incubate with AEC substrate (3-amino-9-ethylcarbazol) for 12min. 10. Wash in PBS11. Counterstain with Mayer's hemalum

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Rekonstitution: Restore with 0.5 mL distilled water (= 0.2 mg/mL stock solution).

Konzentration: 0.2 mg/mL

Buffer: Stock solution contains PBS, pH 7.2 with 0.02 % Sodium Azide as preservative and 10 mg/mL BSA as stabilizer.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: All human source materials have tested negative for HIV1, HIV2, HBc, HCV and HBsAg antibodies. No test guarantees a product to be non-infectious. Therefore, all material derived from human fluids or tissues should be considered as potentially infectious.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: RT/4 °C/-20 °C

Informationen zur Lagerung: Lyophilized: Store at 2-8 °C (preferably in a dessicator). Reconstituted: Aliquot and store at -20 °C.

Details zu Transferrin Receptor

Target: Transferrin Receptor (TFRC) (Transferrin Receptor (p90, CD71) (TFRC))

Andere Bezeichnung: CD71 / TFRC (TFRC Produkte)

Synonyme: Trfr antikoerper, LOC100219280 antikoerper, tfr antikoerper, cd71 antikoerper, tfr1 antikoerper, trfr antikoerper, CD71 antikoerper, T9 antikoerper, TFR antikoerper, TFR1 antikoerper, TR antikoerper, TRFR antikoerper, p90 antikoerper, 2610028K12Rik antikoerper, AI195355 antikoerper, AI426448 antikoerper, AU015758 antikoerper, E430033M20Rik antikoerper, Mtvr-1 antikoerper, Mtvr1 antikoerper, transferrin receptor antikoerper, TFRC antikoerper, Tfrc antikoerper, tfrc antikoerper

Hintergrund: CD71 is a homodimeric membrane glycoprotein consisiting of two disulfide-bonded 95kD chains. It is a type II membrane protein with a short aminoterminal cytoplasmic domain of 61 amino acid residues which mediates internalization and recycling, a single transmembrane region of 28 amino acid residues, and a large extracellular transferrin-binding domain of 671 residues. Other significant structural features include N-linked and O-linked glycans and the covalent attachment of palmitic acid to cystein residues close to the cytoplasmic face of the membrane.Synonyms: TfR1, Transferrin receptor protein 1, p90

Gen-ID: 9606

UniProt: P02786

Pathways: Transition Metal Ion Homeostasis