SLC3A2 Antikörper (FITC)

Produktnummer ABIN1112235

Produktdetails anti-SLC3A2 Antikörper (FITC)

Target: SLC3A2 (Solute Carrier Family 3 (Activators of Dibasic and Neutral Amino Acid Transport), Member 2 (SLC3A2))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser SLC3A2 Antikörper ist konjugiert mit FITC

Applikation: Flow Cytometry (FACS), Immunofluorescence (IF)

Produktmerkmale: Monoclonal Mouse Anti-Human CD98 FITC is recommended for use in flow cytometry for identification of human leukocyte lineages.

Klon: FG1-8

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: It is recommended for use in flow cytometry. This reagent is effective for direct immunofluorescence staining of human tissue for flow cytometric analysis using 20 µl/10^6 cells.

Kommentare:

Fluorescein isothiocyanate (Molecular Probes).

Probennahme: 1. Transfer 100 µl of anticoagulated (EDTA) blood to a 12 x 75 mm polystyrene test tube (10^6 cells). 2. Add 20 µl of CD98 FITC and mix gently with a vortex mixer. The 20 µl is a guideline only, the optimal volume should be determined by the individual laboratory. 3. The recommended negative control is a non-reactive FITC-conjugated antibody of the same isotype. 4. Incubate in the dark at room temperature at 4°C for 30 minutes or at room temperature (20-25 °C) for 15 minutes. 5. Add 1,5 ml of Lysing Solution to each sample and mix gently with a vortex mixer. Incubate for 10 minutes at room temperature in the dark. 6. Centrifuge at 1000 x g for 5 minutes. Gently aspirate the supernatant and discard it leaving approximately 50 µl of fluid. 7. Add 2 ml 0.01 mol/l PBS (It betters that it containing 2% bovine serum albumin) and resuspend the cells by using a vortex mixer. 8. Centrifuge at 1000 x g for 5 minutes. Gently aspirate the supernatant and discard it leaving approximately 50 µl of fluid. 9. Resuspend pellet in an appropriate fluid for flow cytometry, e.g. 0.3 ml PBS. The PBS should contain 1% paraformaldehyde (fixative) if samples are not analysed the same day. 10. Analyse on a flow cytometer or store at 2-8 °C in the dark until analysis. Samples can be run up to 24 hours after lysis.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: The conjugate is provided in liquid form in buffer containing 1% bovine serum albumin (BSA) and 0,09% Sodium azide, pH 7.2.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: 1. The device is not intended for clinical use including diagnosis, prognosis, and monitoring of a disease state, and it must not be used in conjunction with patient records or treatment. 2. This product contains Sodium azide (NaN3), a chemical highly toxic in pure form. At product concentrations, though not classified as hazardous, Sodium azide may react with lead and copper plumbing to form highly explosive build-ups of metal azides. Upon disposal, flush with large volumes of water to prevent metal azide build-up in plumbing. 3. As with any product derived from biological sources, proper handling procedures should be used.

Lagerung: 4 °C

Details zu SLC3A2

Target: SLC3A2 (Solute Carrier Family 3 (Activators of Dibasic and Neutral Amino Acid Transport), Member 2 (SLC3A2))

Andere Bezeichnung: CD98 (SLC3A2 Produkte)

Synonyme: 4F2 antikoerper, 4F2HC antikoerper, 4T2HC antikoerper, CD98 antikoerper, CD98HC antikoerper, MDU1 antikoerper, NACAE antikoerper, Mdu1 antikoerper, MGC53951 antikoerper, slc3a2 antikoerper, MGC53965 antikoerper, SLC3A2 antikoerper, AI314110 antikoerper, Cd98 antikoerper, Ly-10 antikoerper, Ly-m10 antikoerper, Ly10 antikoerper, Mgp-2hc antikoerper, fc65h12 antikoerper, wu:fc65h12 antikoerper, id:ibd1327 antikoerper, sb:cb519 antikoerper, wu:fe14a07 antikoerper, wu:fq18d12 antikoerper, zgc:55813 antikoerper, solute carrier family 3 member 2 antikoerper, solute carrier family 3 (amino acid transporter heavy chain), member 2 L homeolog antikoerper, solute carrier family 3 (amino acid transporter heavy chain), member 2 S homeolog antikoerper, solute carrier family 3 (activators of dibasic and neutral amino acid transport), member 2 antikoerper, solute carrier family 3 (amino acid transporter heavy chain), member 2a antikoerper, solute carrier family 3 (amino acid transporter heavy chain), member 2b antikoerper, SLC3A2 antikoerper, Slc3a2 antikoerper, slc3a2.L antikoerper, slc3a2.S antikoerper, slc3a2a antikoerper, slc3a2b antikoerper

Hintergrund: This antibody recognizes the 125 kDa heterodimeric membrane glycoprotein that is constituted by an 85 kDa heavy chain and a 40 kDa light chain. CD98 is up-regulated on leukocytes and in inflammatory lesions, and strongly expressed by neoplastic cells. However, expression is not hematopoietic specific.