CD42b Antikörper (PerCP)

Produktnummer ABIN1112145

Produktdetails anti-CD42b Antikörper (PerCP)

Target: CD42b (GP1BA) (Glycoprotein Ib (Platelet), alpha Polypeptide (GP1BA))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser CD42b Antikörper ist konjugiert mit PerCP

Applikation: Flow Cytometry (FACS), Immunofluorescence (IF)

Produktmerkmale: Monoclonal Mouse Anti-Human CD42b PerCP is recommended for use in flow cytometry for identification of platelets and megakaryocytes.

Klon: HIP1

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: It is recommended for use in flow cytometry. This reagent is effective for direct immunofluorescence staining of human tissue for flow cytometric analysis using 20 µl/10^6 cells.

Kommentare:

Peridin-cholophyll-protein complex (Prozyme).

Aufbereitung der Proben:

1. Centrifuge tube of freshly drawn EDTA blood (600rpm or 75xg, 20') 2. Remove platelets (top layer) and wash twice over in PBS and resuspend in PBS. 3. Add 20 µl of CD42a PE and mix gently with a vortex mixer. The 20 µl is a guideline only, the optimal volume should be determined by the individual laboratory. 4. The recommended negative control is a non-reactive PE-conjugated antibody of the same isotype. 5. Incubate in the dark at room temperature at 4°C for 30 minutes or at room temperature (20-25 °C) for 15 minutes. 6. Add 2 ml 0.01 mol/l PBS (It betters that it containing 2% bovine serum albumin) and resuspend the cells by using a vortex mixer. 7. Centrifuge at 1000 x g for 5 minutes. Gently aspirate the supernatant and discard it leaving approximately 50 µl of fluid. 8. Analyse on a flow cytometer.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: The conjugate is provided in liquid form in buffer containing 1% bovine serum albumin (BSA) and 0,09% Sodium azide, pH 7.2.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: 1. The device is not intended for clinical use including diagnosis, prognosis, and monitoring of a disease state, and it must not be used in conjunction with patient records or treatment. 2. This product contains Sodium azide (NaN3), a chemical highly toxic in pure form. At product concentrations, though not classified as hazardous, Sodium azide may react with lead and copper plumbing to form highly explosive build-ups of metal azides. Upon disposal, flush with large volumes of water to prevent metal azide build-up in plumbing. 3. As with any product derived from biological sources, proper handling procedures should be used.

Lagerung: 4 °C

Details zu CD42b

Target: CD42b (GP1BA) (Glycoprotein Ib (Platelet), alpha Polypeptide (GP1BA))

Andere Bezeichnung: CD42b (GP1BA Produkte)

Synonyme: BDPLT1 antikoerper, BDPLT3 antikoerper, BSS antikoerper, CD42B antikoerper, CD42b-alpha antikoerper, DBPLT3 antikoerper, GP1B antikoerper, GPIbA antikoerper, VWDP antikoerper, GP1BA antikoerper, GPIba antikoerper, GPIbalpha antikoerper, glycoprotein Ib platelet alpha subunit antikoerper, glycoprotein Ib (platelet), alpha polypeptide antikoerper, glycoprotein 1b, alpha polypeptide antikoerper, GP1BA antikoerper, Gp1ba antikoerper

Hintergrund: The antibody is directed against the CD42b antigen, platelet glycoprotein gp1bA, that serves as a receptor for Von Willebrand factor (vWF) and as a high affinity thrombin receptor. AK2 immunblots a band of 130kDa on SDS gels of platelet lysates and gives surface immunofluorescence with fixed or unfixed platelets. The antigen is absent in very low levels on platelets with the Bernard-Soulier syndrome.