RIF1 Antikörper (AA 2432-2444)

Produktnummer ABIN1103996

Produktdetails anti-RIF1 Antikörper

Target: RIF1 (RAP1 Interacting Factor Homolog (RIF1))

Bindungsspezifität: AA 2432-2444

Reaktivität: Human, Maus

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser RIF1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA

Spezifität: Human RIF1. This antibody is expected to recognize both reported isoforms (NP_060621.3, NP_001171134.1). The immunizing peptide sequence has been optimised for cross-reactiviy in mouse. Reported variants represent identical protein: NP_001171135.1, NP_001171136.1, NP_001171134.1

Homologie: Percent identity by BLAST analysis: Human, Chimpanzee, Orangutan, Gibbon, Monkey, Mouse, Xenopus (93%) Galago, Rat, Panda, Bovine, Dog, Porcine, Guinea pig (86%).

Aufreinigung: Immunoaffinity purified

Immunogen: Synthetic peptide C-RNLQSRWRSPSHEN from the C-terminus of human RIF1 (NP_060621.3, NP_001171134.1). Percent identity by BLAST analysis: Human, Chimpanzee, Orangutan, Gibbon, Monkey, Mouse, Xenopus (93%), Galago, Rat, Panda, Bovine, Dog, Porcine, Guinea pig (86%).

Type of Immunogen: Synthetic peptide

Anwendungsinformationen

Applikationshinweise: Approved: Peptide-ELISA (1:8000), WB (1 - 3 μg/mL)

Usage: Peptide ELISA: antibody detection limit dilution 1:8000. Western blot: Approx 270kD band observed in lysates of cell line HeLa and of Mouse Embryonic Stem cells (calculated MW of 274kD according to NP_060621.3). The observed band is suppressed by introduction of Rif1 siRNA to HeLa. Recommended concentration: 1-3 μg/mL.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Minimize freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Store at -20°C. Avoid freeze-thaw cycles.

Details zu RIF1

Target: RIF1 (RAP1 Interacting Factor Homolog (RIF1))

Andere Bezeichnung: RIF1 (RIF1 Produkte)

Synonyme: 5730435J01Rik antikoerper, 6530403D07Rik antikoerper, AU016181 antikoerper, AW549474 antikoerper, D2Ertd145e antikoerper, RGD1562474 antikoerper, fc38c03 antikoerper, wu:fc38c03 antikoerper, si:dkey-89p3.2 antikoerper, replication timing regulatory factor 1 antikoerper, RAP1 interacting factor homolog (yeast) antikoerper, RIF1 antikoerper, Rif1 antikoerper, rif1 antikoerper

Hintergrund: Name/Gene ID: RIF1

Synonyms: RIF1

Gen-ID: 55183

NCBI Accession: NP_060621, NP_001171134

UniProt: Q5UIP0

Pathways: Stem Cell Maintenance